建立幽门螺杆菌感染BALB/c小鼠动物模型

目的在普通饲养条件下用云南cagA+、vacA+的毒力单株3-21建立小鼠动物模型。方法口服0.2 mol/LNaHCO3,15 min后用胃管灌喂H.Pylori3-21菌株5次,1周内连续完成。灌胃后4周处死动物,用胃黏膜匀浆液进行细菌培养实验、尿素酶实验、胃病理组织学检查,并应用RFLP-PCR法确定动物小鼠感染的H.Pylori为定植所用的菌株。结果灌胃4周后,处死的10只BALB/c小鼠经细菌培养实验、尿素酶实验、胃病理组织学检查均感染上H.Pylori。而对照组动物的胃黏膜匀浆液经各项检测均为阴性。结论成功地建立了H.Pylori感染的小鼠实验动物模型,为H.Pylori致病机制的研究、疫苗开发研制等奠定了一定基础。

The establishment of BALB/c mouse model with Helicobacter pylori infection

Objective In common breeding condition the animal model is established by the Yunnan H.pylori strain on the BABL/c mice.Methods Taking 0.2 mol/L NaHCO3 orally,after 15 minutes we pour the mice H.Pylori 3-21 strain five times continually,and finished in a week.After 4 weeks of infection,all the mice were killed,and three pieces of the gastric mucosa were obtained from pyloric potion,one was used for rapid urease testing,one for histopathological detections,and the rest one was used for PCR-RFLP is used to confirm if the infected strain is the pre-strain which is fed into the mice’ mouths.Results After 4 weeks of in fection,the killed mice were confirmed be infected H.Pylori by the histopathological detections,rapid urease testing and culture of H.pylori.The normal control group was negative.Conclusions The animal model,BABL/c mice of infection is established by Yunnan H.pylori strains carrying cagA genes and vacA gene successfully.