激光捕获显微切割技术应用研究新进展

对疾病发生过程中的组织损害要进行分子水平分析,首要步骤就是纯净目标细胞的分离。近年发展起来的激光捕获显微切割(1aser capture microdissection,LCM)技术可以在显微镜直视下快速、准确地获取所需的单一细胞亚群,甚至单个细胞,从而成功解决了组织中细胞异质性问题。本文介绍了LCM技术的原理及其优缺点,并对其在DNA分析和基因表达分析两个方面的应用研究新进展进行综述,同时对LCM技术的发展和完善提出了可能的方向。

New developments in application of laser capture microdissection

Isolation of pure targeted cells is an important and essential step for the molecular analysis of tissue lesion occurred in the progression of disease. Laser capture microdissection (LCM) is a novel technique that can be applied to obtain pure targeted cell subgroup or even a single cell quickly and precisely under the microscope, thus the problem of tissue heterogeneity in molecular analysis can be tackled successfully. In this article, the principles, advantages and disadvantages of LCM were introduced. New developments in the application of LCM were summarized in two fields (DNA analysis and gene expression analysis) respectively. Meanwhile, possible directions of the future developments of LCM were put forward.