L-精氨酸逆转一氧化氮抑制后的犬冠脉血流动力学改变和内皮素-1含量升高 |
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引用本文: | 刘庆军,王跃民,臧益民,吴明媚. L-精氨酸逆转一氧化氮抑制后的犬冠脉血流动力学改变和内皮素-1含量升高[J]. 心脏杂志, 1996, 0(4) |
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作者姓名: | 刘庆军 王跃民 臧益民 吴明媚 |
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作者单位: | 解放军第91医院心内科,第四军医大学生理学教研室,唐都医院心内科 |
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摘 要: | 观察了犬冠脉内灌注N-单甲基左旋精氨酸(L-NMMA)后再灌注L-精氨酸(L-Arg)和单独灌注L-NMMA前后冠脉血流动力学、冠脉血流储备以及冠脉对不同浓度的乙酰胆碱(Ach)反应的变化,同时用放免法测定冠脉前降支(LAD)伴行静脉血中内皮素-1(ET-1)含量。结果发现,L-Arg完全逆转了灌注L-NMMA引起的血流动力学改变,使心率回升,下降的基础冠脉流量(CBF),从20±8ml/min回升至28±7ml/min,P<0.05),降低的冠脉储备恢复,从51±10ml/min升至94±15ml/min,P〈0.01),ET-1的含量不再升高,从15.5±3.0ng/L下降至5.0±2.0ng/L,P〈0.01),Ach介导的CBF增加不再受到抑制(P〈0.01)。结果提示提供外源性L-Arg可增加一氧化氮(NO)的产生,使由于NO抑制而产生的血流动力学改变和ET-1升高发生逆转。
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关 键 词: | 冠状动脉循环;L-精氨酸;一氧化氮;内皮素;血液动力学;狗 |
Effects of intracoronary L arginine on vasomotion and endothelium dependent responses of coronary arteries and endothelin 1 levels after inhibition of nitric oxide in coronary circulation in anesthetized dogs |
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Abstract: | Peak reactive hyperemic coronary blood flow ( CBF )following 15 s occlusion,resting CBF and CBF in response to acetylcholine(Ach) were observed both before and after a 5 mg / kg intracoronary infusion of N monomethly L arginine(L NMMA) and a 66 mg/ kg of L arginine(L Arg) in open chest anesthetized dogs,Meanwile,serial endothelin 1(ET 1) level was also obtained from the left interventricular vein and measured by radioimmuniassay. Results: L Arg completely reversed heart rate,resting CBF and CBF, and peak reactive hyperemic CBF was decreased by L NMMA. The increase in left interventricular vein blood ET 1 induced by L NMMA were not observed any more for 60min after L NMMA and L Arg infusion. L Arg also reversed the attenuated Ach stimulated endothelium dependent responses toward the levels before L NMMA infusion. These results suggested that a supply of exogenous L Arg could increase nitric oxide product,and reversed the change of hemodynamics and increased ET 1 levels after inhibition of nitric oxide. |
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Keywords: | coronary circulation L arginine nitric oxide endothelin hemodynamics dogs |
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