胃黏膜癌变过程中RRM1和CDX2表达及相关性

目的:检测RRM1、CDX2在不同胃黏膜组织中的表达,探讨其临床意义.方法:收集胃癌90例,高级别上皮内瘤变、低级别上皮内瘤变、肠上皮化生、浅表性胃炎、正常胃黏膜各30例,共240例.采用S-P免疫组化方法对所有组织检测RRM1、CDX2表达,并进行分析.结果:(1)胃癌、高级别上皮内瘤变、低级别上皮内瘤变组RRM1的阳性表达率分别为86.7%、83.3%、83.3%,均高于浅表性胃炎组的66.7%(P<0.05)和肠上皮化生组的33.3%(P<0.01),浅表性胃炎组66.7%高于肠上皮化生组的33.3%(P<0.05);高级别上皮内瘤变、肠上皮化生、胃癌组的CDX2的阳性表达率分别为76.7%,63.3%、61.1%,均高于低级别上皮内瘤变组的33.3%(P<0.05)和浅表性胃炎组的0(P<0.01).(2)210例不同胃黏膜病变中,RRM1与CDX2二者之间表达呈正相关(rs=0.352,P=0.000),90例胃癌中RRM1与CDX2二者之间呈正相关关系(rs=0.223,P=0.034).结论:RRM1、CDX2表达均与胃癌的发生发展有关,二者可作为较为理想的肿瘤标志物,用于胃癌早期诊断的预警.

Expression of RRM1,CDX2 in Carcinogenesis of Gastric Mucosa and their Correlation

Objective:To detect the expression of RRM1 and CDX2 in different gastric mucosa lesions and esplore its clinical significance.Methods:Expression of RRM1 and CDX2 was detected in 6 groups of different gastric tissues 90 cases of gastric cancer,30 cases of high-grade intraepithelial neoplasia,30 cases of low-grade intraepithelial neoplasia,30 cases of intestinal metaplasia,30 cases of superficial gastritis,30 cases of normal gastric mucosa using immunohistochemistry SP method by the correlation analysis.Results:(1)The positive expression rate of RRM1 in gastric cancer group,high-grade intraepithelial neoplasia group and low-grade intraepithelial neoplasia group were 86.7%,83.3% and 83.3%,respectively.Any one of the was higher than at superficial gastritis group which was 66.7%(P<0.05) and at intestinal metaplasia group which was 33.3%(P<0.01).The 66.7% of superficial gastritis group was higher than 33.3% of intestinal metaplasia group(P<0.05);The positive expression rate of CDX2 in high-grade intraepithelial neoplasia group,intestinal metaplasia group and gastric cancer group were 76.7%,63.3% and 61.1%,respectively.Any one of the was higher than at low-grade intraepithelial neoplasia group which was 66.7%(P<0.05) and superficial gastritis group which was 0(P<0.01).(2)The expression of RRM1 in 210 cases of different Gastric Mucosa Lesions was positively correlated with CDX2(rs=0.352,P=0.000,P<0.01).The expression of RRM1 In 90 cases of gastric cancer was positively correlated with CDX2(rs=0.223,P=0.034,P<0.05).Conclusion:The expression of RRM1 and CDX2 is related to the development of gastric cancer.Hence,both of them can be used as ideal tumor markers for early diagnosis of gastric cancer.