15,16-Dihydrotanshinone I, a major component from Salvia miltiorrhiza Bunge (Dansham), inhibits rabbit platelet aggregation by suppressing intracellular calcium mobilization |
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Authors: | Jung-Woo Park Sung-Hun Lee Min-Kyu Yang Jung-Jin Lee Min-Jin Song Si-Yong Ryu Hun-Jong Chung Hyung-Sik Won Chang-Soo Lee Suk-Hyung Kwon Yeo-Pyo Yun Wahn-Soo Choi Hwa-Sup Shin |
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Institution: | (1) College of Pharmacy, Chungbuk National University, Cheongju, 361-763, Korea;(2) Medicinal Chemistry Research Team, Korea Research Institute of Chemical Technology, Daejeon, 305-600, Korea;(3) Pediatric Department, Chungju Hospital, Konkuk Medical School, Konkuk University, Chungju, 380-701, Korea;(4) Department of Biotehcnology, Division of Life Science, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea;(5) Rexgene Biotech Co. Ltd, Seoul 137-070, Korea;(6) College of Medicine, Konkuk University, Chungju, 380-701, Korea;(7) Department of Applied Biochemistry, Division of Life Science, College of Biomedical and Health Science, Konkuk University, 322 Danwol-Dong, Chungju, Chungbuk, 380-701, Korea |
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Abstract: | Radix Salviae miltiorrhiza (RSM, ‘Dansham’ in Korea, ‘Danshen’ in Chinese), the root of Salviae miltiorrhiza Bunge (Labiate)
has been used as Chinese fork medicine for the treatment of cardiovascular diseases such as angina pectoris, coronary heart
disease, myocardial infarction, and hypertension. In the present study, we evaluated the inhibitory effects of 15, 16-Dihydrotanshinone
I, one of the major ingredients of Salvia miltiorrhiza Bunge, on platelet aggregation, with elucidation of its mechanisms
of action. 15,16-Dihydrotanshinone I concentration-dependently inhibited collagen-induced aggregation of rabbit washed platelets
with IC50 of 8.7±5.6 μM, the potency being about seven-fold greater than EGCG, an active Green tea catechin component (IC50: 56.6±48.7 μM). 15,16-Dihydrotanshinone I significantly inhibited the intracellular calcium (Ca2+]i) mobilization in a concentration-dependent manner. 15,16-dihdydrotanshinone I also significantly suppressed collagen (50
μg/mL)-induced liberation of 3H]Arachidonic acid from 3H]Arachidonic acid-incorporated rabbit platelet. In addition, 15,16-Dihydrotanshinone I at 50 μM slightly but significantly
inhibited collagen-induced production of thromboxane B2. These results indicate that 15,16-Dihydrotanshinone I exert potent anti-platelet activity via suppression of Ca2+]i mobilization and arachidonic acid liberation. |
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Keywords: | 15 16-Dihydrotanshinone I Dansham Salviae Washed rabbit platelet Collagen Calcium |
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