AT1R 1166A／C和CYP11B2-344C／T基因多态性与2型糖尿病肾病的关系

目的研究2型糖尿病患者AT1受体基因1166A／C（ATIR1166A／C）与醛固酮合酶（CYP11B2）基因-344C／T（CYP11B2—344C／T）多态性与糖尿病肾病（DN）的相关性。方法研究对象分为DN组73例，糖尿病非肾病（non-DN）组72例及正常对照（NC）组52名。应用聚合酶链反应-限制性内切酶片断长度多态性（PCR—RFLP）方法检测各组基因多态性。结果DN组AT1R 1166A／CAC基因型和C等位基因频率高于Non-DN组（P均〈0．05）。DN组ATlR1166A／C各基因型临床指标比较，AC基因型患者的BUN、Cr、UA、UAER、SBP和DBP均明显高于AA基因型患者。2型糖尿病患者AT1R 1166A／C多态性与DN相关（P〈0．05），但CYP 11B2—344C／T多态性并未发现与DN相关。结论蚌埠地区汉族人群存在AT1R 1166A／C多态性，其AC基因型与糖尿病肾病有显著相关性。

The relationship between polymorphism of AT1R1166A/C and CYP11B2-344C/T gene and diabetic nephropathy in type 2 diabetes mellitus

Objective To explore the association of polymorphism of angiotensinⅡ type 1 receptor （AT1R） gene 1166A/C and aldosterone synthase（CYP11B2） gene-344C/T with type 2 diabetes nephropathy development. Methods DN group,non-DN group and normal control were included. PCR- RFLP methods were used. Results The frequencies of the AC genotype and C allele of AT1R 1166A/C in DN were higher than those in non-DN （all P〈0.05）. The frequencies of T allele of CYP11B2--344T/ C in DN were higher than those in non-DN or NC （P〉0.05）. The clinical indexes of Cr,BUN,uric acid （UA）,UAER,SBP,DBP of AC genotype of AT1R 1166A/C were significantly higher than those of AA genotype in DN. The polymorphism of AT1R 1166A/C was correlated with diabetic nephropathy in type 2 diabetes mellitus（P〈0. 05）, however the correlation with the polymorphism of CYP11B2- 344C/T was not found in DN（P〉0. 05）. Conchtsions The polymorphism of AT1R 1 166A/C exists in Han nationality of Bengbu city and AC genotype is significantly correlated with diabetic nephropthy in type 2 diabetes.