两面针中化学成分的分离鉴定及活性测定

目的研究两面针根的化学成分,寻找具有抗炎活性的先导化合物。方法采用硅胶、反相ODS、Sephadex LH-20等柱色谱以及高效液相色谱等手段分离纯化,并通过理化性质和波谱数据鉴定化合物的结构;利用酶标仪测定吸光度,计算样品的一氧化氮(NO)释放抑制率;MTT法测定细胞存活率。结果从该植物中分离得到13个化合物,分别被鉴定为茵芋碱(skimmianine,1)、白鲜碱(dictamnine,2)、zanthobungeanine(3)、zanthodioline(4)、N-methylflindersine(5)、鹅掌楸碱(liriode-nine,6)、L-芝麻素(L-sesamin,7)、β-香树脂醇(β-amyrin,8)、豆甾-9(11)-烯-3-醇(stigmast-9,9)、十三烷胺(tridecane amine,10)、十四烷胺(tetradecane amine,11)、十七烷胺(heptadecanoic amine,12)、十九烷胺(nonadecane amine,13)。结论化合物3、4、8、10～13均为首次从两面针植物中分离得到;体外活性测试结果表明,化合物3具有温和的NO释放抑制活性,其IC50为37.26 mg.L-1。

Isolation, identification and activity determination on the anti-inflammatory components of roots of Zanthoxylum nitidum

Objective To study the chemical constituents of roots of Zanthoxylum nitidum(Roxb.)DC. and find compounds with anti-inflammatory activity. Methods Silica gel column chromatography, RP-ODS, Sephadex LH-20 and high performance liquid chromatography were applied for the isolation and purification of the constituents. The structures were elucidated by their physicochemical properties and spectral methods., The inhibitory activity on NO production and cell survival was evaluated by MTT assay through measuring the absorbance using microplate reader. Results Thirteen compounds were isolated, including skimmianine(1), dictamnine(2), zanthobungeanine(3)，zanthodioline(4)，N-methylflindersine(5)，liriodenine(6)，L-sesamin(7)，β-amyrin(8)，stigmast-9(11)-en-3-ol(9)，tridecane amine(10)，tetradecane amine(11)，heptadecanoic amine(12) and nonadecane amine(13). Conclusion Compounds 3, 4, 8, 10, 11, 12 and 13 are all isolated from Zanthoxylum nitidum for the first time. Compound 3 shows moderate NO production inhibitory activity with an IC₅₀ value of 37.26 mg·L^-1.