| 西瑞香素与大鼠血浆蛋白结合率的测定 |
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| 引用本文： | 佟立今,郑 玲,张 石,王 晶,孙立新. 西瑞香素与大鼠血浆蛋白结合率的测定[J]. 沈阳药科大学学报, 2013, 30(2): 109-113 |
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| 作者姓名： | 佟立今 郑 玲 张 石 王 晶 孙立新 |
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| 作者单位： | （1. 沈阳药科大学 药学院，辽宁 沈阳 110016；2. 沈阳药科大学 本溪药物研究院，辽宁 本溪 117004） |
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| 基金项目： | 国家“十一五”“重大新药创制”科技重大专项资助项目(2009ZX09301-012) |
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| 摘 要： | 目的采用HPLC法对西瑞香素与大鼠血浆蛋白结合率进行研究。方法采用平衡透析法,结合HPLC法测定西瑞香素的大鼠血浆蛋白结合率。结果西瑞香素在低(0.50 mg.L-1)、中(1.0 mg.L-1)、高(2.0 mg.L-1)3个质量浓度下,其血浆蛋白结合率分别为(91.7±1.8)%、(91.6±1.4)%和(90.7±0.81)%。结论西瑞香素具有较强的血浆蛋白结合率。
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| 关 键 词： | 西瑞香素 血浆蛋白结合率 高效液相色谱法 平衡透析法 |
| 收稿时间： | 2012-04-22 |
| 修稿时间： | 2012-12-22 |
Determination of the binding rate of rat plasma protein with daphnoretin |
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| TONG Li-jin,ZHENG Ling,ZHANG Shi,WANG Jing,SUN Li-xin. Determination of the binding rate of rat plasma protein with daphnoretin[J]. Journal of Shenyang Pharmaceutical University, 2013, 30(2): 109-113 |
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| Authors: | TONG Li-jin ZHENG Ling ZHANG Shi WANG Jing SUN Li-xin |
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| Affiliation: | 1,2(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Benxi Institute of Pharmaceutical Research,Shenyang Pharmaceutical University,Benxi 117004,China) |
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| Abstract: | Objective To study the rat plasma protein binding rate, an accurate and specific high performance liquid chromatography method (HPLC) for quantifying daphnoretin in rat plasma was developed．Methods The equilibrium dialysis combined with HPLC was carried out for the determination of the plasma concentration and plasma protein binding rate of daphnoretin. Results Rat plasma protein binding rates of daphnoretin at low, middle and high concentrations (0.50, 1.0, 2.0 mg·L-1) were (91.7 ± 1.8) %, (91.6 ± 1.4) %, (90.7 ± 0.81) %, respectively. Conclusion Daphnoretin has high binding power to rat plasma protein. |
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| Keywords: | daphnoretin plasma protein binding rate HPLC equilibrium dialysis |
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