| RNA干扰技术抗肠道病毒71型复制 |
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| 引用本文： | 李国栋,杨 倬,张景海.RNA干扰技术抗肠道病毒71型复制[J].沈阳药科大学学报,2013,30(1):57-62. |
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| 作者姓名： | 李国栋 杨 倬 张景海 |
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| 作者单位： | 1. 沈阳药科大学 生命科学与生物制药学院，辽宁 沈阳 110016；2. 中国科学院微生物研究所 病原微生物与免疫学重点实验室，北京 100101 |
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| 摘 要： | 目的肠道病毒71型(Enterovirus 71,EV 71)是手足口病(hand,foot and mouth disease,HFMD)主要的病原体之一。研究RNA干扰技术抑制肠道病毒71型复制。方法以RNA干扰技术(RNAi)为干预手段,抑制病毒EV 71在人横纹肌肉瘤(rhabdomyosarcoma,RD)细胞中的复制。通过Western blot、Real-time PCR和病毒滴度3种方法验证,其抑制效果体现在感染细胞内部病毒RNA,病毒蛋白质的表达水平和培养基上清中子代病毒颗粒的数量上。结果靶向病毒基因组5'UTR和VP2的siRNAs具有明显的病毒抑制效应。结论此研究证实RNAi方法具有特异性抑制病毒复制的潜能和可行性。
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| 关 键 词： | RNA干扰 肠道病毒71型 抑制 |
| 收稿时间： | 2012-5-8 |
| 修稿时间： | 2012-7-8 |
RNA interference against Enterovirus 71 replication |
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| LI Guo-dong,YANG Zhuo,ZHANG Jing-hai.RNA interference against Enterovirus 71 replication[J].Journal of Shenyang Pharmaceutical University,2013,30(1):57-62. |
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| Authors: | LI Guo-dong YANG Zhuo ZHANG Jing-hai |
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| Institution: | 1. School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Center for Molecular Virology, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Objective To provided certain scientific basis to suppress Enterovirus 71 (EV 71) replication by RNA interference (RNAi). Method RNA interference (RNAi) was used as a therapeutic approach to inhibit EV 71 replication in rhabdomyosarcoma (RD) cells. The inhibition effects were confirmed by a corresponding decrease in viral RNA of the infected cells, the expression level of viral protein and the number of progeny virus in the cultured supernatants. The inhibitory effect was validated through Western-blot, Real-time PCR and virus titer. Results The siRNAs targeting viral genome 5 ’UTR and VP2 could effectively block viral replications. Conclusion This study confirms that RNAi method possesses potential and feasibility in the specific viral inhibition. |
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| Keywords: | RNA interference Enterovirus 71 inhibition |
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