Preventive effects of intermittent administration of human parathyroid hormone on steroid-induced osteopenia in rats |
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Authors: | Naohisa Miyakoshi |
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Institution: | (1) Department of Orthopedic Surgery, Akita University School of Medicine, 1-1-1 Hondo, 010 Akita, Japan |
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Abstract: | The purpose of this study was to determine the preventive effect of intermittent administration of human parathyroid hormone
(h-PTH) on bone change in steroid-treated rats; this was done by histomorphometric and biochemical analysis. Seven-month-old
female Wistar rats were divided into four groups; in-each of the four groups one subgroup was treated for 4 weeks and one
for 8 weeks. The groups consisted of: untreated controls, a steroid group (receiving prednisolone), a steroid + PTH group
(predniso-lone and h-PTH administered simultaneously), and a steroid + PTH vehicle group. Prednisolone (2.5 mg/kg) and h-PTH
(1–34) (6.0 μg/kg) were administered six times a week during the experimental period. At necropsy, bilateral tibiae were collected:
one was used for preparing undecalcified sections after Villanueva bone staining, and the other for decalcified tartrate-resistant
acid phosphatase (TRAP) stained sections. Biochemical analysis showed that steroids increased urinary calcium at the 8th week;
however, such bone metabolic markers as serum 1,25-(OH)2D and urinary deoxypyridinoline did not change in any treatment group. Histomorphometrically, steroid-induced osteopenia was
established at the 8th week by inhibition of both bone formation and bone resorption. The simultaneous intermittent administration
of PTH plus steroid, however, increased both bone formation and bone resorption, resulting in increases in bone volume beginning
at 4 weeks. These results suggest that the simultaneous intermittent administration of PTH with steroid prevents steroid-induced
low-turnover osteopenia by stimulating bone turnover. |
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Keywords: | human parathyroid hormone steroid-induced osteopenia bone histomorphometry |
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