人胆管癌裸鼠移植瘤2号模型的建立

目的 建立人胆管癌裸鼠移植瘤模型。方法 将人胆管癌组织接种于裸鼠皮下和肝脏，逐代观察移植瘤生长情况，进行形态学和生物学特性鉴定。结果 建立了人胆管中分化乳头状腺癌裸鼠移植瘤2号模型，命名为HBDCM2—ZSH。已连续传代7代，皮下移植瘤生长率为100％。潜伏期21d，成瘤时间为6周左右。移植瘤在形态和生物学上仍保持人胆管癌的特点。结论 HBD-CM2-ZSH裸鼠移植瘤模型是一种接近人体的胆管癌模型，可为胆管癌研究提供动物模型。

Establishment of No.2 implanted tumor model of human cholangiocarcinoma in nude mice

Objective To establish a model of implanted tumor model of human cholangiocarcinoma in nude mice. Methods Surgical specimens from patients with cholangiocarcinoma were implanted into the subcutaneous layer and liver of nude mice. Growth and morphological and biological features of the implanted tumor were observed at each passage. Results A moderately differentiated capillary adenocarcinoma model of human cholangiocarcinoma in nude mice was established and named by HBDCM2-ZSH. It had been maintained for 7 passages in nude mice. The growth rate of subcutaneous implantation was 100%, latent period 21 d and time for tumor formation about 6 weeks. Implanted tumors exhibited the characteristics of the original tumor in morphology and biology. Conclusions HBDCM2-ZSH is a model similar to human cholangiocarcinoma and provides an animal model for research of cholangiocarcinoma.