重组人血管内皮抑制素对鼻咽癌HNE-1细胞株体外血管生成拟态的抑制作用

目的：研究重组人血管内皮抑制素（rh-Endostatin，Endostar，恩度）对人鼻咽低分化鳞癌HNE-1细胞的增殖、迁移及体外血管生成拟态的影响。方法：用不同浓度恩度（0～50μg／m1）处理HNE-1细胞，MTT法检测HNE-1细胞增殖活性的变化；创伤修复实验检测细胞迁移能力的变化。在Matrigel上接种HNE-1细胞，观察HNE-1细胞能否形成血管网状结构及其特点；体外管状形成抑制试验检测恩度对HNE-1细胞管道形成能力的影响。结果：恩度（1-50μg／m1）组的OD值与对照组比较无明显统计学差异（P〉0．05），但可以显著降低HNE-1细胞的迁移速度（P〈0．01），且与浓度呈负相关（r=-0．983，P〈0．01）。HNE-1细胞在Matrigel上培养能形成血管网状样结构，并能与内皮细胞连接共同构成马赛克样血管网状结构；恩度能抑制HNE。1细胞体外管道形成（P〈0．01），其管状结构数量与恩度浓度呈负相关（r=-0．978，P〈0．01）。结论：HNE-1细胞具有血管生成拟态及与内皮细胞共同形成马赛克血管的能力，恩度能够抑制HNE-1细胞的迁移和体外血管生成拟态形成。

Inhibitory effect of rh-endostatin on vasculogenic mimicry in nasopharyngeal cancer cell line HNE-1 in vitro

Objective：To investigate the effect of rh-endostatin （endostar） on the proliferation, migration and vasculogenic mimicry in poor-differentiated human nasopharyngeal cancer cell line HNE-1 in vitro. Methods：HNE-I cells were cultivated in culture medium with endostar in the concentrations ranging from 0 to 50μg/ml, and the effect on proliferation and migration were detected by MTT assay and wound healing assay in vitro, respectively. HNE-1 cells were seeded on the surface of matrigel which allowed them to attached and observed whether they could form the capillary tube-like structures（TLSs） ; Cultured HNE-1 cells were devided into endostar group and control group, and the effect on tube formation of HNE-1 cells were detected by tube-like structure formation assay. Results ：OD of endostar group was not significantly lower than the control group in the concentrations ranging from 1 to 50μg/ml（ P 〉 0. 05 ） , the migration velocity of HNE-l cells in endostar group was significantly lower than the control group （ P 〈0. 01 ） ; HNE-1 cells could form TLSs and mosaic vessels when mix-cultured with HUVECs on matrigel, the number of tubules in endostar group were significantly lower than the control group （ P 〈 0. 01 ）. Conclusion ： Nasopharyngeal cancer cell line HNE-1 is capable of forming tube-like structures and mosaic vessels when mix-cultured with HUVECs. Endostar can inhibit their migration and vasculogenic mimicry formation in vitro.