毛细管气相色谱法测定雪抑乐中龙脑的含量

目的:建立以毛细管气相色谱法测定雪抑乐中龙脑含量的方法。方法:色谱柱为SPBTM-1毛细管柱(30m×0.25mm×0.25μm),检测器为氢火焰离子化检测器(FID),柱温为100℃,进样器温度为235℃,检测器温度为245℃,载气为高纯氮气,流速为1.2mL.min-1,分流比为1∶10,内标为萘。结果:龙脑检测浓度在0.0464～0.2320mg.mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9991);平均回收率为96.62%,RSD=0.84%(n=6)。结论:本方法简便、快速、准确,可用于雪抑乐的质量控制。

Determination of Borneol in Xueyile by Capillary GC

OBJECTIVE:To establish a capillary GC method for the determination of Borneol in Xueyile.METHODS:The determination was performed on SPBTM-1 capillary column(30m× 0.25mm× 0.25μ m)with flame ionization detector(FID).The temperatures of column,injector,and detector were 100℃,235℃,and 245℃ respectively.The carrier gas was N2;the flow rate stood at 1.2mL· min-1 and the split ratio at 1∶ 10.The internal standard was naphthalene.RESULTS:The linear range for Borneol was 0.046 4 ～ 0.232 0mg· mL-1(r=0.999 1).The average recovery was 96.62%(RSD=0.84%,n=6).CONCLUSION:The method is simple,rapid,and accurate,and it can be applied to the quality control of Borneol.