胃癌前疾病不同中医证型IL-1、IL-8、IL-10基因多态性分布评价

目的:探讨胃癌前疾病不同中医证型中IL-1、IL-8、IL-10基因多态性的分布情况。方法:招募85例慢性萎缩性胃炎、胃溃疡或胃息肉患者及20名健康志愿者,抽取外周血作为检测样本,采用等位基因专一性实时荧光PCR技术检测IL-1B-31 C/T及IL-1B-511 T/C、IL-8-251 T/A、IL-10-592 C/A及IL-10-1082 A/G基因位点SNP并进行基因分型;分析基因分型在不同中医证型及健康对照组中的分布差异,并评价各基因型与胃癌发病风险的关系。结果:IL-1B-31 TT基因型和IL-10-1082 AG基因型在脾胃湿热组中较健康对照组明显增多(P0.05),IL-8-251 AA基因型在肝胃不和证和脾胃虚弱证中较对照组显著增高(P0.05),而IL-1B-511 T/C、IL-10-592 C/A各基因型在各组及证型中的分布无显著性差异。结论:不同胃癌前疾病中医证型IL-1、IL-8、IL-10基因型分布不尽相同,提示不同中医证型与基因多态性可能有一定的相关性;携带有IL-1B-31 TT和(或)IL-10-1082 AG型基因的脾胃湿热证,或携带有IL-8-25 AA型基因的肝胃不和及脾胃虚弱证胃癌前疾病患者可能患胃癌的风险增大。

Distribution of gene polymorphisms of IL-1, IL-8 and IL-10 in gastric precancerous diseases with different TCM syndromes

  Objective: To explore the distribution of gene polymorphisms of IL-1, IL-8 and IL-10 in gastric precancerous diseases with different TCM syndromes.   Methods: 85 patients with chronic atrophic gastritis, gastric ulcer or gastric polyps and 20 healthy volunteers were recruited. The peripheral blood was taken as test samples. The single nucleotide polymorphisms(SNP)of IL-1B-31 C/T and IL-1B-511 T/C, IL-8-251 T/A, IL-10-592 C/A and IL-10-1082 A/G loci were detected by allele-specific real-time fluorescence PCR,and then the genotyping was performed. The distribution differences of genotypes in different TCM syndromes and healthy controls were analyzed, and the relationship between genotypes and risk of gastric cancer was evaluated.   Results: The IL-1B-31 TT genotype and IL-10-1082 AG genotype in the syndrome of damp-heat in spleen and stomach were obviously more than those in the healthy control(P<0.05),the IL-8-251 AA genotype in the syndrome of incoordination between liver and stomach and syndrome of deficiency of spleen and stomach was significantly more than that in the healthy control(P<0.05). For the IL-1B-511 T/C genotype and IL-10-592 C/A genotype,there were no significant differences on the distribution between different groups and different syndromes.   Conclusion: The distribution of IL-1, IL-8 and IL-10 genotypes in different TCM syndromes of gastric precancerous diseases is different,which suggests that there may be correlation between different TCM syndromes and gene polymorphisms. The patients of gastric precancerous diseases with syndrome of damp-heat in spleen and stomach who carrying IL-1B- 31 TT and (or) IL-10-1082 AG genotypes,or the patients with syndrome of incoordination between liver and stomach and syndrome of deficiency of spleen and stomach who carrying IL-8-25 AA genotypes,may have an increased risk of gastric cancer.