HPLC法同时测定人参固本丸中人参皂苷Rg1和人参皂苷Re的含量

目的 建立了测定人参固本丸中的有效成分人参皂苷Rg1和人参皂苷Re的高效液相色谱测定方法.方法 采用UltimateTM XB-C18柱(250mm×4.6mm 5μm);乙腈-0.1%磷酸溶液(20∶80)为流动相;检测波长为203nm;流速1.0mL·min-1;柱温35℃.结果 人参皂苷Rg1在0.0752μg~1.8800μg具有良好的线性关系(r=0.9996),平均回收率为97.8%,RSD为0.011%(n=9);人参皂苷Re在0.1887μg~4.7175μg的范围具有良好的线性关系(r=0.9992),平均回收率为98.2%,RSD为0.015%(n=9).结论 本法快速、准确,可同时测定人参固本丸中人参皂苷Rg1、人参皂苷Re的含量.

Determination of Ginsenoside Rg1 and Ginsenoside Re in Renshen Guben Pills by HPLC

OBJECTIVE To establish for the content determination of ginseno side Rg1 and ginsenoside Re in Renshen Guben pill.METHODS A sp herisorb C18 colum(250mm×4.6mm 5μm)was used as the analytical column an d acetonitrile-0.1% phosphoric acid (20∶80) as the mobile phase;T he detectio n wavelength was at 203nm.The flow rate was 1.0mL·min-1 RESULTS The calibration curves of ginsenoside Rg1 and ginsenoside Re were line ar in the range 0.0752μg~1.8800μg (r=0.9996) and 0.1887μg~4.7175μg (r=0.9992) respectively.The average recoveries were 97.8%,(RSD=0.011%,n=9)fo r ginsenoside Rg1 and 98.2%(RSD=0.015%,n=9) for ginsenoside Re.CONC LUSION The method is simple,rapid and accurate,and the result is reli able.