| 建立稳定表达融合自杀基因CD／UPRT．UL49的CNE-2鼻咽癌细胞株 |
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| 引用本文： | 卿菁,赵素萍,蒋卫红,章华. 建立稳定表达融合自杀基因CD／UPRT．UL49的CNE-2鼻咽癌细胞株[J]. 临床耳鼻咽喉头颈外科杂志, 2014, 0(3): 178-181 |
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| 作者姓名： | 卿菁 赵素萍 蒋卫红 章华 |
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| 作者单位： | 中南大学湘雅医院耳鼻咽喉科长沙,410008 |
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| 基金项目： | 基金项目：国家自然科学基金（No：30672296） |
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| 摘 要： | 目的：建立稳定表达融合自杀基因CD／UPRT．UL49的CNE-2鼻咽癌细胞株。方法：构建表达载体pcDNA3．1（一）E6．BARFlP．cD／UPRT．UL49，经脂质体法转染CNE-2细胞，G418和前体药物5-氟胞嘧啶筛选出阳性克隆细胞并扩大培养。抽提阳性克隆细胞总蛋白质，Western-blotting检测目的基因表达。结果：融合自杀基因CD／UPRT．UL49在CNE2转染细胞内稳定表达。结论：本组通过脂质体转染技术，成功地建立了稳定表达融合CD／UPRT．UL49基因的CNE-2细胞株。
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| 关 键 词： | 自杀基因 CD UPRT UI。49 CNE-2细胞 鼻咽肿瘤 |
Construction of nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49 |
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| QING J ing ZHAO Suping,J IANG Weihong,ZHANG Hua. Construction of nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49[J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 2014, 0(3): 178-181 |
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| Authors: | QING J ing ZHAO Suping J IANG Weihong ZHANG Hua |
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| Affiliation: | (Department of Otolaryngology, Xiangya Hospital of Central South University, Changsha, 410008,China) Corresponding author ： QING Jing, E-mail. angelqingjing@ hotmail, com |
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| Abstract: | Objective：To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UIA9. Method：The plasmids of pcDNA3. 1 （一）E6. BARFlp. CD/UPRT. UIA9 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UIA9 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE 2 cells. Result： Suicide genes were expressed stably in CNE-2 cells. Conclusion： We con- structed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamin. |
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| Keywords: | suicide gene CD/UPRT. UI.49 CNE-2 cells nasopharyngeal neoplasm |
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