慢性中耳炎细菌生物膜形成与细菌培养结果的相关性分析

目的：探讨慢性中耳炎细菌生物膜形成与细菌培养之间的相关性。方法：对32例慢性化脓性中耳炎及中耳胆脂瘤患者术中取得的样本进行扫描电镜检查，并对中耳分泌物行细菌培养；分析慢性中耳炎细菌生物膜形成与细菌培养结果之间的关系。结果：慢性化脓性中耳炎（活动期）与中耳胆脂瘤细菌生物膜形成率分别为87．5％、81．3％（P〉0．05）。慢性中耳炎扫描电镜细菌生物膜结果与细菌培养结果之间比较，灵敏度为70．37％，特异度为60.00％，误诊率为40．00％，漏诊率为29．63％，阳性预测值为90．46％，阴性预测值为27．27％，正确率为68．75％，约登指数为30．37％，Pearson相关系数为0．232（P〉0．05）。结论：慢性化脓性中耳炎（活动期）与中耳胆脂瘤均有较高的生物膜形成率，但中耳分泌物常规细菌培养结果不能反应慢性中耳炎患者细菌生物膜的形成情况，需要探索更为可靠的细菌学实验方法来准确反映慢性中耳炎的感染情况。

Correlation analysis of bacterial biofilm formation and bacterial culture in chronic otitis media

Objective：To study the correlation between the bacterial biofilm formation and bacterial culture in chronic otitis media.Method：As a prospective reserch,we used scanning electron microscopy to examinate patients＇ samples which collected from 32cases of patients with chronic suppurative otitis media and middle ear cholesteatoma in the operations,and performed the middle ear secretions bacterial culture.According to the different types of chronic otitis media group,we analysised the relationship between chronic otitis media bacterial biofilm formation and the bacterial culture results.Result：Chronic suppurative otitis media（activity）and middle ear cholesteatoma bacterial biofilm formation rate were 87.5%,81.3%,chi-square（P〉0.05）.Compared bacterial biofilm results with the results of bacterial cultured in chronic otitis media,sensitivity was 70.37%,specificity was60.00%,the misdiagnosis rate was 40.00%,the missed diagnosis was 29.63%,positive predictive value was90.46%,negative predictive value was 27.27%,accuracy was 68.75%,Youden index was 30.37%,and Pearson correlation coefficient was 0.232（P〉0.05）.Conclusion：Chronic suppurative otitis media（activity）and middle ear cholesteatoma bacteria had a higher biofilm formation rate.The routine bacterial culture results can′t reflecte bacterial biofilm formation in chronic otitis media.We need to explore more reliable experimental methods to accurately reveal the infection status of chronic otitis media.