氢氧化铝佐剂对变应性鼻炎小鼠模型的影响

目的：研究氢氧化铝佐剂对变应性鼻炎（AR）小鼠模型的影响，探讨AR小鼠建模的恰当方法。方法：采用卵清蛋白（0VA）鼻内致敏、鼻内激惹（局部干预组）和腹腔内OVA加用氢氧化铝Al（OH）3佐剂注射致敏、OVA鼻内激惹（系统干预组）2种方法建立BALB／c小鼠AR模型，动态观察2种模型小鼠打喷嚏数和挠鼻次数；应用苏木精-伊红染色形态学观察小鼠鼻黏膜上皮内杯状细胞和黏膜下腺体增生情况；Luna染色形态学观察2种模型小鼠鼻黏膜内嗜酸粒细胞的浸润并对其计数。以酶联免疫吸附试验（ELISA）检测2种模型小鼠鼻腔冲洗液和血清中IL-4、IL-5、OVA特异性IgE（sIgE）和IFN-γ的浓度。结果：局部干预组小鼠打喷嚏数和挠鼻次数显著高于系统干预组。苏木精-伊红染色观察前者小鼠鼻黏膜上皮内杯状细胞和黏膜下腺体增生较后者显著；Luna染色前者鼻黏膜内嗜酸粒细胞计数也显著高于后者。局部干预组鼻腔冲洗液和血清中IL-4、IL-5和sIgE的浓度明显高于系统干预组，而其IFN—γ的浓度明显低于系统干预组。结论：OVA和AI（OH）。佐剂联合经腹腔注射致敏可以同时促进Th1和Th2型细胞免疫反应，OVA局部鼻内致敏、鼻内激惹是建立AR小鼠模型的恰当方法。

Influence of aluminum hydroxide adjuvant on a murine model of allergic rhinitis

Objective To investigate the influence of aluminum hydroxide adjuvant on a murine model of allergic rhinitis （AR） and to confirm an appropriate method of establishing a mouse model of AR. Method： Establishing two types of BALB/c mice models of AR, one was identified as Local group which was characterized through intranasal sensitization and challenge using ovalbumin （OVA）, and the other Systemic group which was made by intraperitoneal sensitization with OVA plus aluminum hydroxide and intranasal challenge through OVA. Then the numbers of sneezing and nasal rubbing were counted after the last challenge and the eosinophils in the nasal mucosa of mice models were observed and counted though Luna stain. Furthermore, morphological hyperplasia was examined in intraepithelial goblet cells and s ubmucosal glands with HE stain. In addition, interlukin （IL） -4, IL-5, OVA specific IgE （sIgE） and interferon （IFN） -γ in nasal iavage fluid （NLF） and serum of mice were examined using enzyme linked immunosorbent assay （ELISA）. Result：The counts of sneezing and nasal rubbing in local group were more than those in systemic group and eosinophilia in the nasal, mueosa of former group was greater than that in the latter one. Morphological hyperplasia was stronger in intraepithelial goblet cells and submucosal glands in local group compared with that in systemic group. Furthermore, the contents of IL-4, IL-5 and sIgE increased in the NLF and serum of mice of local group compared to those of systemic one. However, the production of IFN-γ of mice in local group decreased when compared with that in Systemic group. Conclusion：OVA plus aluminum hydroxide adjuvant may promote Thl type immune response as well as Th2 response. OVA intranasal sensitization and challenge locally is an appropriate way in the establishment of AR mice models.