肿瘤相关巨噬细胞和基质溶解素在喉鳞状细胞癌组织的表达与肿瘤微血管密度的关系

目的：探讨CD68抗体标记的肿瘤相关巨噬细胞TAMs和基质溶解素MMP-7在喉鳞状细胞癌组织的表达及与临床病理参数的关系,探讨两种分子标志物表达与喉癌组织MVD的关系。方法：使用免疫组织化学法检测标本65例（喉鳞状细胞癌组织45例,瘤旁非肿瘤组织20例）中CD68及MMP-7、CD34抗体标记的MVD的表达。结果：CD68阳性表达率,鳞癌组织（82.2%,37/45）与瘤旁组织（15.0%,3/20）比较,差异有统计学意义（P〈0.05）。MMP-7阳性率鳞癌组织（71.1%,32/45）与瘤旁组织（25.0%,5/20）比较,差异有统计学意义（P〈0.05）。CD34-MVD鳞癌组织（26.52±6.36）高于瘤旁组织（12.23±4.01）。有淋巴结转移组CD68、MMP-7阳性率高于无淋巴结转移组;MMP-7、CD68表达与分化程度无关;MMP-7与肿瘤分期无关,而CD68与肿瘤分期相关;CD68、MMP-7与CD34-MVD有正相关性。结论：两种分子标志物在喉癌组织的高表达及与MVD的正相关性说明了TAMs与MMP-7在促进喉鳞状细胞癌组织的转移及新生血管形成中发挥了作用,可作为判断喉癌侵袭及转移的重要标志物。

The expression of tumor related macrophages and matrix solution element in laryngeal squamous carcinoma tissue and the relationship with tumor microvascular density

Objective：To study the expression of CD68 antibody marked tumor associated macrophage TAMs and matrix solution element MMP-7 in laryngeal squamous carcinoma tissue and the relationship with elinicopatho- logical parameters, so that to expore the relationship between the expression of the two molecular markers and la- ryngeal cancer tissue mierovascular density（MVD）. Method： Immunohisto chemical method was employed to detect the expression of CD68 and MMP-7 in 65 cases（laryngeal squamous carcinoma tissue in 45 cases; peritumoral non- tumor tissue in 20 cases） and CD 34 antibody marked MVD expression. Result：CD68 positive rate in squamous car- cinoma tissue（82.2 ~/00,37/45） is obviously higher than that in the periturnoral tissue（ 15 ~, 3/20） （P~0.05） ,and MMP-7 positive rate in squarnous carcinoma tissue is significantly different from that in peritumoral tissue （71, 1~ ;25~）（P~0.05）. The expression rate o{ CD34-MVD in laryngeal squamous carcinoma tissue（ 26.52_＋ 6.36 ）is higher than that in peritumoral tissue （12.23_＋4.01）（P~0.05）. In lymph node metastasis group, the positive expression rates of CD68 and MMP-7 are higher than those in the group without lymph node metastasis. MMP-7 showed no correlation with cancer stage, and CD68 was related with cancer stage; CD68, MMP-7 and CD34- MVD have positive correlation. Conclusion：The high level of expression of TAMs and MMP-7 in laryngeal cancer tissue and the positive correlation with MVD illustrate that both of the markers play important roles in pro- moting laryngeal squamous carcinoma tissue metastasis and angiogenesis, which can be used as important markers to evaluate the invasion and metastasis of laryngeal cancer.