天然免疫蛋白PLUNC在鼻息肉中的表达及与鼻息肉临床特征的关联性

目的：探讨天然免疫蛋白腭、肺、鼻上皮克隆（PLUNC）在鼻息肉组织中的表达情况，分析PLUNC蛋白浓度与鼻息肉大小（鼻内镜记分）和手术后复发的关联性。方法：采用免疫组织化学和实时定量PCR检测鼻息肉组（28例鼻息肉患者，其中13例为术后复发患者）和对照组（16例钩突黏膜对照）PLUNC的组织染色定位和mRNA的表达情况，同时采用ELISA检测初发和复发鼻息肉组织中PLUNC的蛋白浓度差异，评估PLUNC蛋白浓度与息肉大小、鼻塞、流涕症状记分的相关性。结果：鼻息肉组织中PLUNC主要定位在黏膜上皮和腺体，染色强度记分显著低于对照的钩突组织（P〈0．01）；PLUNCmRNA表达水平也显著低于对照的钩突组织（P〈0．01）。初发和复发鼻息肉组织中PLUNC蛋白OD值分别为0．33±0．11和0．15±0．05，差异有统计学意义（P〈0．01）。小型和大型鼻息肉PLUNC蛋白OD值分别为0．32±0．14和0．19±0．07，差异有统计学意义（P〈0．05）。鼻息肉组织中PLUNC蛋白浓度与鼻塞和流涕记分存在显著负相关（r=-0．51和r=-0．57，P〈0．01）。结论：PLUNC的表达降低提示鼻息肉的发生可能与天然免疫反应的减弱有关，因此，上调天然免疫分子如PLUNC等的表达可作为干预鼻息肉发病的一个新策略。

Expression of palate,lung, and nasal epithelial clone in primary and recurrent nasal polyps

Objective：To determine the expression o{ palate, lung, and nasal epithelial clone （PLUNC） in nasal polyps （NP） and evaluate its association with clinical severity. Method： Twenty-eight NP patients （primary polyp, 15; recurrent polyp, 13） and 16 normal controls （healthy uncinate process） were enrolled, the expression of PLUNC was examined in nasal tissues by immunohistochemistric staining, quantitative PCR and ELISA respectively. The protein level of PLUNC in nasal polyps was correlated with nasal symptom score （nasal congestion and rhinorrhea, respectively）. Result：PLUNC was mainly distributed in the epithelial layer and submucosal glands in nasal tissues. The staining intensity and mRNA level of PLUNC were significantly decreased in polyp tissues than in normal controls （P〈0.01）. The protein levels of PLUNC were 0. 33±0.11 and 0.15±0.05 in primary and recurrent polyp tissues （P〈0.01）, and were 0. 32±0.14 and 0.19±0.07 in small-size and big-size polyp tissues （P〈0.05）. The protein level of PLUNC in polyp tissues significantly correlated with both nasal congestion score and rhinorrhea score （r=-0.51 and r=-0.57, P〈0.01）. Conclusion：Decreased PLUNC in polyp tissues indicated that impaired innate immunity may account for the pathogenic process of NP. Thus upregulating PLUNC may represent a promising therapeutic target for the management of NP.