| 枸杞多糖的提取分离和其组成研究 |
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| 引用本文： | 田丽梅,王旻. 枸杞多糖的提取分离和其组成研究[J]. 中国中药杂志, 2006, 31(19): 1603-1607 |
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| 作者姓名： | 田丽梅 王旻 |
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| 作者单位： | 中国药科大学,生命科学与技术学院,江苏,南京,210009 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 目的：从枸杞中提取分离枸杞多糖(LBP),并对其组成进行研究。方法：采用水提法提取LBP,用DEAE纤维素柱色谱和凝胶柱色谱进行分离纯化,采用GPC-LLS法、气相色谱法、氨基酸自动分析等方法对其组成进行研究。结果：LBP纯品含有4个级分,LBP的分子质量约为1.524×105,由阿拉伯糖(Ara),鼠李糖(Rha),木糖(Xyl),甘露糖(Man),半乳糖(Gal)和葡萄糖(Glc)6种中性单糖和半乳糖醛酸及18种氨基酸组成,结论：LBP是酸性杂多糖同多肽或蛋白质构成的的复合性多糖,且存在Glycan-O-Ser的糖肽结构。
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| 关 键 词： | 枸杞多糖 提取 分离 组成 |
| 文章编号： | 1001-5302（2006）19-1603-05 |
| 收稿时间： | 2005-11-05 |
| 修稿时间： | 2005-11-05 |
Studies on extraction, isolation and composition of Lycium barbarum polysaccharides |
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| TIAN Meili;WANG Min. Studies on extraction, isolation and composition of Lycium barbarum polysaccharides[J]. China Journal of Chinese Materia Medica, 2006, 31(19): 1603-1607 |
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| Authors: | TIAN Meili WANG Min |
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| Affiliation: | School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China. |
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| Abstract: | OBJECTIVE: To study the extraction, isolation and composition of Lycium barbarum polysaccharides (LBP). METHOD: LBP was extracted from L. barbarum with water, isolationed and purified by DEAE ion-exchange cellulose and gel chromatography, and their structural composition was studied by means of SDS-PAGE gel electrophoresis, GC, amino acid automatic analysis, etc. RESULT: Pure LBP has four water solubie polysaccharides, M W was 1.524 x 10(5). LBP was composed of 6 kinds of monosaccharides (Ara, Rha, Xyl, Man, Gal and Glc), galacturonic acid and 18 kinds of amino acids. CONCLUSION: LBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein, and LBP has Glycan-O-Ser glycopeptide structures. |
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| Keywords: | Lycium barbarum polysaccharide extraction isolation composition |
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