抗纤软肝冲剂抗肝纤维化的实验研究

目的 :通过抗纤软肝冲剂对肝纤维化动物防治作用的研究 ,探讨该方抗肝纤维化的作用机理。方法 :四氯化碳、高脂、高胆固醇、低蛋白等复合因素诱导大鼠肝纤维化动物模型 ,同时给予抗纤软肝冲剂 ,以秋水仙碱为对照。检测肝组织羟脯氨酸 Hyp、超氧化物歧化酶 SOD及丙二醛 MDA,观察血清肝纤维化指标 (透明质酸 HA、层粘蛋白 LN、 型前胶原 PC 、 型胶原 -C)及肝功能 (白蛋白 Alb、球蛋白 G、谷丙转氨酶 A LT)。结果 :抗纤软肝冲剂对肝纤维化大鼠有升高A lb,降低 AL T和 G的作用 ;显著提高肝 SOD活性 ,降低 MDA含量 ;显著降低 HA ,LN ,PC , -C;减轻肝脏 Hyp的作用。结论 :抗纤软肝冲剂具有确切的防治肝纤维化的作用 ,其机理在于抗脂质过氧化和保护肝细胞 ,对胶原代谢也有直接作用

Experimental studies of Kangxian Ruangan Granule on Anti-hepatic fibrosis in rats

Objective:It is to observe the effect and mechanism of Kangxian Ruangan Granule(KQRGG) on the experimental hepatic fibrosis in rats.Methods: The model of hepatic fibrosis was induced by complex factors of CCl 4 and foods of high fat and lower protein, and treated with KQRG.Colchicine treatment was taken as control group. Hyp, SOD and MDA of liver tissue, serum fibrotic items of HA, LN, PCⅢ and Ⅳ-C, and serum Alb, G and ALT were examined. Results: KQRGG could remarkably raise Alb content, decrease G content and ALT activity, improve SOD activity and lower MDA content significantly, drcrease the level of HA, LN, PCⅢ, Ⅳ-C, and Hyp content evidently. Conclusion:KQRGG had a good anti-hepatic fibrosis effect by inhibiting lipoperioxidation, protecting HC, and adjusting collagen metabolism.;