腺苷治疗大鼠脊髓损伤的初步研究

目的 通过观察大鼠脊髓损伤后内源性细胞外腺苷含量的变化以及外源性腺苷对脊髓损伤后脊髓组织细胞外钙离子和神经功能的影响,探讨腺苷在脊髓继发性损伤中的作用。 方法 ＳＤ大鼠５８只,制作成Ｔ１３脊髓腹侧压迫模型,用微透析技术每２０ｍｉｎ连续收集脊髓损伤后脊髓组织细胞外液,用高效液相色谱法仪紫外检测法检测细胞外液腺苷的含量;伤前１５ｍｉｎ蛛网膜下给予非特异性腺苷受体激动剂２－氯腺苷,伤后立即开始每１０ｍｉ

Adenosine in treatment of rats with spinal cord injury

Objective To observe the changes of endogenous extracellular adenosine after spinal cord injury (SCI) to rats and the effect of exogenous adenosine on extracellular calcium after SCI and post injury neurolo gical function. Methods A ventral compression injury model of T13 spinal cord was used, and the extracellular fluids were collected consecutively every 20 minutes after injury by using microdialysis. Adenosine in the samples was analyzed using high pressure liquid chromatography (HPLC) with u.v. detection. The rats received different doses of 2 chloroadenosine (2 CADO), a nonspecific agonist of adenosine receptors, by intrathecal injection 15 minutes before injury. The extracellular fluid was collected every 10 minutes immediately after injury and the calcium was measured using atomic absorption spectrophotometer. Neurological function score, inclined plane angle, and histology were observed 24 hours after injury. Results A significant increase of adenosine was found immediately after spinal cord injury. The concentration of adenosine peaked at one hour after injury and dropped down to the basal level. There was a positive relation between the increase of adenosine and the severity of SCI. High dose of 2 CADO can significantly significantly inhibit the decrease of extracellular calcium and improve the neurological function of injured rats. Conclusions Adenosine could involve the pathological process of secondary spinal cord injury and might play a protective role in SCI.