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| 中药对血管平滑肌细胞增殖的抑制作用的研究* | |
| 引用本文： | 王威,徐宗佩,刘红隐,刘生.中药对血管平滑肌细胞增殖的抑制作用的研究*[J].天津中医药大学学报,2003,22(2):23-25. |
| 作者姓名： | 王威 徐宗佩 刘红隐 刘生 |
| 作者单位： | 1. 300052,天津医学高等专科学校 2. 300193,天津中医学院 3. 300200,天津肿瘤研究所 4. 300204,天津肿瘤医院 |
| 基金项目： | 河北省自然科学基金课题(397256) |
| 摘 要： | 目的]研究中药抑制血管平滑肌细胞(VSMC)生长增殖的作用和机制.方法]采用3H-TdR(氚胸腺苷)掺入和外源性c-jun cDNA在血管平滑肌细胞中表达的方法,观察血管平滑肌细胞(VSMC)生长增殖变化.结果]与对照血清+内皮素组(cpm 2 534.54±54.2/105细胞)相比,中药血清+内皮素组(cpm 1 104.33±37.31/105细胞)血管平滑肌细胞3H-TdR掺入量有显著下降(P＜0.01),而且血管平滑肌细胞原癌基因的c-jun mRNA表达也较弱.结论]本实验表明复方丹参对血管平滑肌细胞增殖及其原癌基因c-jun mRNA的表达有明显的抑制作用. |
| 关 键 词： | 中药 血管平滑肌细胞 细胞培养 c-jun |
| 文章编号： | 1005-7145(2003)02-0023-03 |
| 收稿时间： | 3/8/2003 12:00:00 AM |
| 修稿时间： | 2003年3月8日 |
Inhibition of Chinese material medica on vascular smooth muscle cell hyperplasia |
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| WANG Wei,XU Zong-pei,LIU Hong-yin.Inhibition of Chinese material medica on vascular smooth muscle cell hyperplasia[J].Journal of Tianjin University of Traditonal Chinese Medicine,2003,22(2):23-25. | |
| Authors: | WANG Wei XU Zong-pei LIU Hong-yin |
| Institution: | Tianjin Medical College, Tianjin 300052, China |
| Abstract: | Objective] To study the effect and mechanism of Chinese material medica on the inhibition of vascular smooth muscle cell (VSMC) hyperplasia. Methods] 3H-TdR incorporation and expression of c-jun cDNA in VSMC was used. The growth and hyperplasia of VSMC were observed. Results] The amount of 3H-TdR incorporation in serum+endothelin-1 with Chinese herbs group (cpm 1104.33±37.31/105 cell) was lowered than that in serum+endothelin-1 group (cpm 2S34.S4±54.22/105 cell) with a significant difference (P<0.01). And the c-jun mRNA expression of carcinoma insitu to was also weaker in the former group. Conclusion] It shows that compound Danshen dripping pills can inhibit the hyperplasia of VAMC and c-jun mRNA expression of carcinoma insitu. |
| Keywords: | c-jun |
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