宫颈疾病患者外周血CD4+CD25+C-D127-/low Treg细胞的检测及临床意义

目的 检测宫颈癌、宫颈上皮内瘤变患者外周血CD4+CD25+CD127-/low Treg细胞的表达,分析其临床意义.方法 荧光抗体CD127-FITC、CD4-PECY5、CD25-PE标记T细胞,流式细胞术测定50例宫颈癌患者和49例宫颈上皮内瘤变患者中CD4+CD25+CD127-/low Treg细胞的表达.结果 与正常对照组n=33,(8.07±2.18)％]比较,宫颈癌组n=50,(11.15±1.97)％]、宫颈上皮内瘤变组n=49,(10.61±2.62)％]CD4+CD25+CD127-low均显著升高,两者比较差异有统计学意义(t=6.018,P＜0.001；t=4.943,P＜0.001)；与宫颈上皮内瘤变Ⅰ级组n=16,(8.96±0.65)％]比较,宫颈上皮内瘤变Ⅱ～Ⅲ级组n=33,(11.54±2.72)％]显著升高,两者比较有差异统计学意义(t=4.114,P＜0.001)；与宫颈癌Ⅰ期组n=20,(10.34±1.79)％]比较,官颈癌Ⅱ期组n=30,(11.68±1.97)％]显著升高,两者比较差异有统计学意义(t=2.424,P＜0.05).结论 外周血CD4+CD25+CD127-/low Treg细胞的表达升高可能在宫颈癌和宫颈上皮内瘤变发病的发生、发展过程中起一定作用.

Detection of the expression of CD4+CD25+CD127-/low Treg cells in peripheral blood in patients with cervix diseases and its clinical significance

Objective To detect the expression of CD4+CD25+CD127-/low Treg cells in peripheral blood in patients with cervix cancer and cervical intraepithelial neoplasia(CIN),and to explore its clinical significance.Methods T cells were marked with fluorescence antibody CD127-FITC,CD4-PECY5,CD25-PE.The levels of CD4+CD25+CD127-/low Treg cells were detected by flow cytometry in 50 patients with cervix cancer(group A),49 patients with CIN(group B),and 33 healthy individuals(the control group).Results Compared with the control group (8.07±2.18)%],the levels of CD4+CD25+CD127-/low Treg cells in group A (11.15±1.97)%] and group B (10.61±2.62)%] were significantly higher(t=6.018,P<0.001;t=4.943,P<0.001).The level of CD4+CD25+CD127-/low Treg cells in patients with CIN of grade Ⅱ~Ⅲ n=33,(11.54±2.72)%] was significantly higher than that in patients with CIN of gradeⅠn=16,(8.96±0.65)%],with t=4.114 and P<0.001.The level of CD4+CD25+CD127-/low Treg cells in patients with cervix cancer of stage Ⅱ n=30,(11.68±1.97)%] was significantly higher than that of patients with cervix cancer of stageⅠ n=20,(10.34±1.79)%],with t=2.424 and P<0.05.Conclusion CD4+CD25+CD127-/low Treg cells in the peripheral blood might play an important role in the development and progression of cervix cancer and CIN.