葡萄球菌的分离鉴定及药敏分析

目的：了解不同种类葡萄球菌在本院临床标本中的感染情况及耐药性，为临床治疗和控制此类感染提供参考。方法：采用VITEK-AMS GPI卡进行鉴定，纸片扩散法(NCCLS)及GPS卡进行药敏实验、β内酰胺酶测定和MRS检测，并结合临床资料进行分析。结果：总共3753株感染菌中822株为葡萄球菌(21．90％)，其中金黄色葡萄球菌(SA)322株(39．17％)，凝固酶阴性葡萄球菌(CNS)500株(60．83％)。SA中293株(90．99％)产生β内酰胺酶，苯唑西林耐药者(MRSA)183株(56．83％)；CNS中450株(90．00％)产生β内酰胺酶，苯唑西林耐药的凝固酶阴性葡萄球菌(MRCNS)350株(70．00％)。结论：葡萄球菌特别是凝固酶阴性葡萄球菌是临床感染的主要致病菌之一；万古霉素是有效治疗苯唑西林耐药的葡萄球菌(MRS)的唯一可选药物；合理使用抗生素对治疗和控制感染至关重要。

Identification and antibiotic resistance of staphylococcus

Objective:To study the role of various species of Staphylococci in the clinical infections and their drug resistance with a view to offering references for the control of the infection.Methods:VITEK AMS GPI card was used for identification,disc diffusion test methods(NCCLS)and GPS card were used to detect the drug resistance,beta lactamase and MRS.Results:Among the 3 753 strains isolated from clinical samples,822( 21.90 %) strains were staphylococci ,among which 322 strains(39.17%) were staphylococci aureur (SA) and 500(60.83%) coagulase negtive staphylococci (CNS).90.99%(293) of SA produced beta lactamase and 56.83 %(183) were MRSA;90.00% of CNS were beta lactamase positive strains and 70.00% were MRCNS.Conclusions: Staphylococci, especially CNS,is one of the main pathogens leading to clinical infections.Vancomycin is the only available effective antibiotic.It is very important to use antibiotics properly for the control of the infections.