| Wnt/Frizzled信号通路靶基因C-myc在肾癌中的生物学意义 |
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| 引用本文： | 林桂亭,臧桐,张志文.Wnt/Frizzled信号通路靶基因C-myc在肾癌中的生物学意义[J].中华泌尿外科杂志,2000,21(10):593-595. |
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| 作者姓名： | 林桂亭 臧桐 张志文 |
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| 作者单位： | 北京大学第一医院泌尿外科,北京大学泌尿外科研究所 |
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| 摘 要： | 目的 探讨Ｃ－ｍｙｃ基因作为Ｗｎｔ／Ｆｒｉｚｚｌｅｄ信号通路靶基因在肾癌生物学行为中的表达极其意义。方法 采用细胞核蛋白印迹（Ｗｅｓｔｅｒｎ－ｂｌｏｔ）、免疫组化及免疫细胞化学Ｃ－ｍｙｃ蛋白半定量分析方法，对３２例肾癌组织及肾癌细胞株ＧＲＣ－Ｉ、ＲＣＣ－９４９进行了研究。结果 Ｃ－ｍｙｃ蛋白半定量分析方法，对３２例肾癌组织及肾癌细胞株ＧＲＣ－Ｉ、ＲＣＣ－９４９进行了研究。结果 Ｃ－ｍｙｃ在肾癌组织
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| 关 键 词： | 肾癌 生物学 Wnt／Frizzled信号通路 C－myc基因 |
Expression of Wnt/Frizzled pathway target gene C-myc in renal cell carcinoma |
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| LIN
Guiting, ZANG Tong, ZHANG Zhiwen,et al..Expression of Wnt/Frizzled pathway target gene C-myc in renal cell carcinoma[J].Chinese Journal of Urology,2000,21(10):593-595. |
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| Authors: | LIN
Guiting ZANG Tong ZHANG Zhiwen |
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| Institution: | LIN
Guiting, ZANG Tong, ZHANG Zhiwen,et al.
Urology Institute of Beijing University,Beijing 100034,China |
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| Abstract: | Objective To investigate the expression and function of Wnt/Frizzled pathway target gene C myc. Methods 32 cases with renal carcinoma and renal cancer cell strains (RCC 949 and GRC I) were studied by lumi light western blotting of nuclear crude extract and immunohistochemical staining. Results The expression of C myc was positive in renal cell carcinoma, the positive rate being 90.6%(29/32) and 75.0%(24/32) in tumor cell plasma and nuclear respectively. The expression of C myc protein in GRC I cell strain was higher than RCC 949 cell strain. Expression of C myc is related to tissue type and clinical type of renal cell carcinoma. Conclusion C myc ,as a target gene of Wnt/Frizzled pathway, plays important roles in carcinogenesis of renal cancer. It will be an important and valuable target in cellular signal treatment of renal cell carcinoma. |
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| Keywords: | Kidney neoplasms Carcinoma Genes |
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