Wip1在非小细胞肺癌组织及细胞中的表达

目的： 检测肺癌组织和3种肺癌细胞中Wip1的表达水平，探讨肺癌中Wip1的表达水平与其各种临床病理特征之间的关系。方法: 利用实时荧光定量PCR检测44例肺癌及相应正常肺组织中Wip1 mRNA的表达,分析Wip1 mRNA表达与各临床病理参数之间的关系；利用实时荧光定量PCR的方法检测人肺腺癌细胞A549、人鳞癌细胞NCI-1299、人大细胞肺癌细胞NCI-H460和人正常支气管上皮细胞HBE中Wip1 mRNA的表达量，进行相对定量分析。结果: 44例非小细胞肺癌及相应正常肺组织均有Wip1 mRNA的表达，其中17例肺癌中Wip1 mRNA高表达，占38.6%，两者表达差异显著(ratio=2.1644±1.3940，P＜0.05)；分化程度低的肿瘤细胞Wip1 mRNA表达量显著高于分化程度高者（P<0.05）。3种肺癌细胞中的Wip1 mRNA表达量显著高于正常支气管上皮细胞,差异显著(均P＜0.05)。结论: Wip1mRNA在非小细胞肺癌中过表达，可能与肿瘤发生有关，有望成为非小细胞肺癌基因治疗的新靶点。Wip1 mRNA在非小细胞肺癌中的表达与肿瘤细胞分化程度有关，可能成为确定肿瘤恶性程度的分子生物学参考指标。

Expression of Wip1 in non small cell lung cancer tissue and cells

AIM: To detect the expression level of wip1 in lung cancer tissue and three lung cancer cell lines, and to explore the relations between the expression level of Wip1 in lung cancer and various clinical and pathological features. METHODS: Real-time PCR was employed to detect the expression of Wip1 mRNA in 44 specimens of non small cell lung cancer tissues and normal tissues. The relations between the expression of Wip1 mRNA and various clinical and pathological features were analyzed. Real-time PCR was also employed to detect the expression of Wip1 mRNA in A549, NCI-1299, NCI-H460 and HBE for relative quantitative analysis.RESULTS: In the 44 specimens, the expressions of Wip1 mRNA in both cancer tissues and normal lung tissues were positive. Wip1 gene was over-expressed in 17 specimens in 44 non small cell lung cancer specimens. The rate was 38.6%. The relative level of Wip1 mRNA in NSCLC tissues was significantly higher than that in normal lung tissues (ratio=2.1644±1.3940, P<0.01). The expression of Wip1 mRNA was also correlated with pathological grading (P<0.05). The relative level of Wip1 mRNA in three kinds of lung cancer cells was significantly higher than that in HBE cells. The difference was statistically significant (P<0.05).CONCLUSION: The Wip1 mRNA is over-expressed in non small cell lung cancer, indicating that Wip1 is related to the tumorigenesis and may become the new target of non small cell lung cancer gene therapy. The expression of Wip1 mRNA is related to tumor cell differentiation and may use for the molecular biological reference index to estimate the malignant degree of cancer.