肾乐胶囊对大鼠5/6肾切除肾衰模型作用机理的实验研究

目的 :通过对大鼠 5/6肾切除 (5/6NX)所致肾衰模型的研究 ,探讨中药复方肾乐胶囊治疗慢性肾衰的作用机理。方法 :采用纤维蛋白原平板溶环法 ,检测尿中纤溶酶原激活物PA的活性 ;用半定量法分析残肾组织中组织型纤溶酶原激活物 ,尿激酶型纤溶酶原激活物 /纤溶酶原激活物抑制物 (tPA ,uPA/PAI-1)的染色强度和面积 ;用Northernblot来观察转化生长因子β(TGF-β)mRNA的表达。 结果 :模型对照组大鼠尿PA活性下降 ,肾组织tPA ,uPA表达下降 ;而肾组织中PAI-1和TGF-βmRNA表达增高。肾乐胶囊治疗后 ,尿PA的活性升高 ,肾组织中tPA ,uPA的表达增强 ;而肾组织中PAI-1和TGF-βmRNA的表达却下降。 结论 :肾乐胶囊可以通过调节 5/6NX模型大鼠尿PA的活性 ,肾组织tPA ,uPA/PAI-1,TGF-βmRNA的表达 ,延缓肾小球硬化和间质纤维化病变的进展。

Curative machanism of Shenle capsule on 5/6 nephrectomy rats

OBJECTIVE: To explore curative machanism of Shenle capsule on the 5/6 nephrectomy rats. METHOD: Fibrin plate method was applied to examine activity of urinary plasminogen activator(PA). Semi-quantitative analysis was used to observe stained intensity and area of tissue-type plasminogen activator, urokinas-type plasminogen activator/ plasminogen activator inhibitor(tPA, uPA/PAI-1)in remnant renal tissue. Northern blot was employed to analyze the expression of transforming growth factor (TGF-beta) mRNA. RESULT: In model control group, the urinary PA activity and protein expression of tPA, uPA were down-regulated, but protein expression of PAI-1, TGF-beta mRNA was up-regulated in remnant renal tissue. In each treated group, the urinary PA activity and protein expression of tPA/uPA were enhanced,but the protein expression of PAI-1, TGF-beta mRNA decreased simultaneously. CONCLUSION: Shenle capsule can delay glomerulosclersis and tubulointerstitial fibrotic lesions of remnant kidney by improving the activity of urinary PA and modulating the expression of tPA, uPA/PAI-1 and TGF-beta mRNA.