A flow cytometric method for the measurement of phagocytosis by polymorphonuclear leucocytes |
| |
| Authors: | R M Wilson A M Galvin R A Robins W G Reeves |
| |
| Affiliation: | 1. Department of Immunology, University of Nottingham, University Park, Nottingham NG7 2UH, U.K.;2. Cancer Research Campaign Laboratories, University of Nottingham, University Park, Nottingham NG7 2UH, U.K. |
| |
| Abstract: | A new method for the measurement of phagocytosis of Candida albicans by human polymorphonuclear leucocytes (PMN) is described using a fluorescence activated cell sorter. We have used acridine orange to discriminate between PMN which have internalised yeast particles and those which have not. This method allows accurate measurement of particle phagocytosis as an event distinct from particle adherence. It also permits detailed examination of the kinetics of phagocytosis, the study of which is likely to be of value in the investigation of diseases where abnormalities of PMN function are suspected. |
| |
| Keywords: | flow cytometry phagocytosis kinetics Candida albicans acridine orange opsonins |
| 本文献已被 ScienceDirect 等数据库收录！ |
