| 抗抗CD3 ScFv单克隆抗体的制备及鉴定 |
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| 引用本文： | 邵晓枫,熊冬生,高灜岱,许元富,郭红星,刘芳,郭桂庆,杨纯正.抗抗CD3 ScFv单克隆抗体的制备及鉴定[J].中国免疫学杂志,2007,23(2):142-145. |
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| 作者姓名： | 邵晓枫 熊冬生 高灜岱 许元富 郭红星 刘芳 郭桂庆 杨纯正 |
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| 作者单位： | 中国医学科学院,血液病医院,血液学研究所,实验血液学国家重点实验室,天津,300020 |
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| 基金项目： | 国家自然科学基金;天津市科技发展基金 |
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| 摘 要： | 目的：制备抗抗CD3 ScFv单克隆抗体并研究其生物活性.方法：分离纯化后的抗CD3 ScFv蛋白免疫BALB/c小鼠,采用传统杂交瘤技术制备抗抗CD3 ScFv单克隆抗体;采用ELISA和Western blot鉴定其亚类和抗原结合特异性;采用FACS测定抗抗CD3 ScFv单克隆抗体对Jurkat细胞特异活性.结果：成功筛选出一株能稳定分泌抗抗CD3 ScFv单克隆抗体的杂交瘤细胞株（10B7）,其分泌的抗体亚类为IgG1.该抗抗CD3 ScFv单克隆抗体直标后可特异性结合抗CD3 ScFv蛋白和抗CD3抗体.结论：文中所研制的抗抗CD3 ScFv单克隆抗体具有与抗CD3抗体、抗CD3 ScFv蛋白特异结合的活性,在肿瘤导向治疗中抗CD3/抗肿瘤双特异抗体的亲和层析纯化、药代动力学监测等方面具有广泛的应用前景.
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| 关 键 词： | 抗CD3 ScFv蛋白 单克隆抗体 杂交瘤 双特异抗体 |
| 文章编号： | 1000-484X（2007）02-0142-04 |
| 修稿时间： | 2006-04-132006-07-20 |
Production of monoclonal antibody against anti-CD3 scFv |
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| SHAO Xiao-Feng,XIONG Dong-Sheng,GAO Ying-Dai,XU Yuan-Fu,GUO Hong-Xing,LIU Fang,GUO Gui-Qing,YANG Chun-Zheng.Production of monoclonal antibody against anti-CD3 scFv[J].Chinese Journal of Immunology,2007,23(2):142-145. |
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| Authors: | SHAO Xiao-Feng XIONG Dong-Sheng GAO Ying-Dai XU Yuan-Fu GUO Hong-Xing LIU Fang GUO Gui-Qing YANG Chun-Zheng |
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| Abstract: | Objective:To prepare monoclonal antibody against anti-CD3 ScFv and study its biological activity.Methods:Anti-CD3 ScFv was used for immunizing BALB/c mice.McAb against anti-CD3 ScFv was prepared by hybridoma technique.ELISA and Western blot were used to identify their immunoglobin subtype and specificity.The binding activities of anti-CD3 ScFv McAb against Jurkat cells was detected by FACS.Results:McAb against anti-CD3 ScFv(10B7)was prepared,which belonged to IgG1.It could react with anti-CD3 antibody specifically.Conclusion:McAb against anti-CD3 ScFv has the specific binding activity with anti-CD3 antibody and anti-CD3 ScFv protein,and therefore be potential for tumor target therapy. |
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| Keywords: | Anti-CD3 ScFv protein McAb Hybridoma Bispecific antibody |
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