Islet1 selectively promotes peripheral axon outgrowth in Rohon‐Beard primary sensory neurons |
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| Authors: | Hideomi Tanaka Yasuhiro Nojima Wataru Shoji Miki Sato Ryoko Nakayama Toshio Ohshima Hitoshi Okamoto |
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| Affiliation: | 1. Laboratory for Developmental Gene Regulation, RIKEN Brain Science Institute (BSI), Saitama, Japan;2. Department of Life Science and Medical Bio‐Science, Waseda University, Tokyo, Japan;3. School of Bionics, Tokyo University of Technology, Tokyo, Japan;4. Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan;5. Research Resources Center, RIKEN BSI, Saitama, Japan |
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| Abstract: | We isolated a novel zebrafish mutant, lullaby (llb), and showed that the llb locus encodes the zebrafish orthologue of isl1. Rohon‐Beard (RB) primary sensory neurons are multipolar neurons that extend their central axons longitudinally within the spinal cord and also extend their peripheral axons under the skin. In llb embryos, the outgrowth of the peripheral axons of RB neurons was selectively impaired, which correlated with down‐regulation of the expression of dihydropyrimidinase‐like 3 (dpysl3, also known as collapsin response mediator protein 4, crmp4). Antisense morpholino oligonucleotide (AMO)‐mediated knockdown of dpysl3 inhibited the outgrowth of the peripheral axons of RB neurons, and semaphorin 3d (sema3d) AMO enhanced this effect. These data indicate that Dpysl3 is cooperating with Sema3d in the peripheral axon outgrowth, and Isl1 is required for the selective outgrowth of the peripheral axons of RB neurons by maintaining the expression of dpysl3. Developmental Dynamics 240:9–22, 2011. © 2010 Wiley‐Liss, Inc. |
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| Keywords: | Islet1 Dpysl3 CRMP4 semaphorin Rohon‐Beard neuron axon guidance zebrafish |
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