米非司酮对人早孕绒毛细胞增殖和凋亡的影响

目的探讨米非司酮对人早孕绒毛滋养细胞增殖和凋亡的影响。方法对１５例正常早孕吸宫流产和１５例米非司酮流产病例的绒毛组织,应用免疫组织化学ＡＢＣ法检测增殖细胞核抗原（ＰＣＮＡ）在细胞中的染色（细胞增殖）情况,ＤＮＡ缺口原位末端标记法检测细胞凋亡。结果正常早孕绒毛细胞滋养细胞增殖指数为５３．７４％±１．３４％,且见细胞浆阳性染色,合体滋养细胞未见ＰＣＮＡ阳性染色;合体滋养细胞和细胞滋养细胞的凋亡指数分别为２．５２％±０．８６％和０．５２％±０．２６％;米非司酮流产绒毛细胞滋养细胞增殖指数降为５０．８６％±１．４４％,细胞浆阳性现象消失;细胞凋亡指数显著增加,合体滋养细胞和细胞滋养细胞分别达到２２．１６％±２．２６％和２０．１２％±１．７４％。结论米非司酮终止早孕可能是通过抑制滋养细胞增殖和促进滋养细胞凋亡来实现的

Effects of mifepristone on proliferation and apoptosis in early chorionic villi

OBJECTIVE: To study the effects of mifepristone on proliferation and apoptosis in early pregnant chorionic villi. METHODS: Proliferation and apoptosis in early pregnant choionic villi from surgical aspiration and mifepristone induced abortion were studied. Marker for proliferation is proliferating cell nuclear antigen (PCNA) immunohistochemical staining, apoptotic cell death was detected using DNA in situ terminal deoxynucleotidyl transferas-mediated dUTP-biotin nick ending labeling (TUNEL). RESULTS: In early pregnant chorionic villi, proliferating index of cytotrophoblasts was 53.74% +/- 1.34%, not only nuclear but also cytoplasma were PCNA positive staining, syncytiotrophoblasts were negative. There existed apoptotic cell death in trophoblasts, the apoptotic index were 2.52% +/- 0.86% in syncytiotrophoblasts and 0.52% +/- 0.26% in cytotrophblasts, respectively. After 2 days mifepristone treatment, the proliferating index of cytotrophoblasts decreased slightly and PCNA positive cytoplasmic expression disappeared; while apoptotic cell increased significantly, the apoptotic indices were 22.16% +/- 2.26% in syncytiotrophoblasts and 20.12% +/- 1.74% in cytotrophoblasts, respectively. CONCLUSION: Mifepristone may inhibit proliferation and promote apoptosis of trophoblasts in early pregnant chorionic villi.