Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization |
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| Authors: | Yoshihiro Kaku Akira Noguchi Glenn A Marsh Jennifer A Barr Akiko Okutani Kozue Hotta Boldbaatar Bazartseren Christopher C Broder Akio Yamada Satoshi Inoue Lin-Fa Wang |
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| Institution: | Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku, Tokyo 162-8640, Japan. ykaku@nih.go.jp |
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| Abstract: | A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2.5 × 10(4) TCID(50). Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay. |
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