高温、噪声对飞行员红细胞膜ATP酶活性的影响

目的 探讨高温、噪声环境中飞行对飞行员红细胞膜ＡＴＰ酶活性的影响。方法 轰六飞行员２４名为实验组，普遍地面人员２１名为对照组。作多靶场轰炸飞行，舱内温度４５～５３℃，噪 ９７～１２２ｄＡ（Ａ）。飞行时间３ｈ，于飞行前（６：００ａ．ｍ．）飞行后即刻（１２：００ａ．ｍ．）、８ｈ（８：ｐ．ｍ）采血。对照组采血日行室内作业（２７℃）。用比色法测定红细胞膜Ｎａ＾＋－Ｋ＾＋ＡＴＰ酶、Ｃａ＾２＋－Ｍｇ＾２＋ＡＴ

Effects of High Temperature and Noise on Erythrocyte Me mbrane ATPase Activity in Pilots During Flight

Objective. To determine the effect of heat and noise on erythrocyte membrane ATPase activities in pilots during flying. Method. Twenty-four pilots performing bombing for 3 h (45-53 degrees C, 122-97 dB in the cabin) served as the subjects. 21 ground personnel served as control (27 degrees C in the room). Blood samples were taken from both groups before flying (6:00 a.m.), and immediately (12:00 a.m.) and 8 h (8:00 p.m.) after flying. Na(+)-K+ ATPase, and Ca2(+)-Mg2+ ATPase activities in erythrocyte membrane were determined with colorimetry. Result. The Na(+)-K+ ATPase activity in erythrocyte membrane at 6:00 a.m. in pilots was higher than that in control group at the same time (P<0.01). The Ca2(+)-Mg2+ ATPase activities in erythrocyte membrane at 12:00 a.m. and 8:00 p.m. in pilots were significantly higher, compared with those in control group at the same time (P<0.01). Conclusion. The ATPase values obtained in our study were all within normal range, and the daytime variation of both groups are the same. Exposure of human body to heat and noise for long time may be harmful, the higher ATPase activity is, the more catabolism of ATP will be. ATP exhaustion will lead to Ca2+ overload in erythrocyte thus stiffen the red cell membrane.