白蛋白减轻大鼠液压颅脑损伤伤后脑水肿和神经功能障碍的剂量效应

目的 探讨白蛋白在治疗大鼠液压颅脑损伤后神经功能恢复和脑组织含水量的剂量效应。方法 将96只SD大鼠随机分为4组,液压打击致伤。伤后30min经尾静脉注射2g/kg(n=24,Ⅰ组)、1.2g/kg(n=24,Ⅱ组)、0.8g/kg(n=24,Ⅲ组)、0.4g/kg(n=24,Ⅳ组)20％人血白蛋白。每组取12只伤前、伤后当日、伤后连续7d进行行走、平衡和记忆实验,记录神经功能恢复情况。另每组12只伤后48h取双侧大脑半球测湿重、干重,计算脑组织含水量。结果 实验Ⅰ组动物的行走实验、平衡实验、记忆均优于其他组(P<0.01)。实验Ⅰ组的脑组织含水量为78.09±0.42％,Ⅱ组为79.01～0.66％,Ⅲ组为78.89±0.45％,Ⅳ组为79.03±0.35％,Ⅰ组脑水肿程度明显低于其它组(P<0.01)。结论 伤后早期使用大剂量白蛋白对大鼠液压颅脑损伤有明显促进神经功能恢复和减轻脑水肿的作用。

Dose-effect of albumin to the reduction of brain edema and the improvement of neurological deficits following fluid percussion brain injury in rats

Objective To investigate the dose-effect of albumin to the reduction of brain edema and the improvement of neurological deficits following fluid percussion brain injury in rats. Methods 96 SD rats, all accepted fluid-percussion injury,were divided into four groups randomly, and 2 g/kg (n=24, group Ⅰ )、1.2 g/kg (n=24, group Ⅱ )、0.8 g/kg (n=24, group Ⅲ)、0.4 g/kg (n=24, group Ⅳ) of 20 % human serum albumin were injected transperiponeally after 30 minutes of percussion injury.Everyday behavioral function was evaluated once before and after injury from day 1 to day 7. Cerebral water content was measured by the dry-wet method at 48 hours after injury. Results Neurobehavioral tests including beam walking test, beam balance test and memory test, showed that neurobehavioral functions were significantly better in group I than in other groups (P＜0.01).Cerebral water content was 78.09±0.42 % in group Ⅰ, 79.01±0.66 % in group Ⅱ, 78.89+0.45 % in group Ⅲ, and 79.03±0.35 % in group Ⅳ (P＜0.01). Conclusion Early application of high dose of albumin significantly improves neurological deficits and reduces brain edema after fluid percussion brain injury in rats.