CHOP/Gadd153在强噪声诱导下豚鼠耳蜗细胞凋亡中的表达和作用

目的:探讨强噪声对豚鼠耳蜗细胞死亡的机制及CHOP/Gadd153在强噪声诱导耳蜗细胞凋亡中的作用.方法:选用健康雄性白色豚鼠32只,将实验组豚鼠暴露在4 kHz窄带噪声120 dB SPL噪声环境中4 h,噪声刺激停止后1,4,14 d组(实验组)及对照组(每组各8只)在处死前测听性脑干反应(ABR).取每组4只豚鼠...

Role of CHOP/GADD153 Experssion in Intense Noise-Induced Cochlea Cells Apoptosis of Guinea Pig

Objective: To investigate the mechanism of intense noise-induced cochlea cells death in guinea pig and the role of CHOP/Gadd153 during the procedure of cochlea cells apoptosis.Methods: Thirty-two guinea pigs were randomly divided into four groups.The guinea pigs in the experiment groups were exposed to 4 kHz narrow band noise at 120 dB SPL for 4 h.After the noise expose of 1,4 or 14 days on the experiment guinea pigs,auditory brainstem response(ABR) of the guinea pigs in experiment and control groups were tested before killed.Apoptosis was tested by terminal deoxynucleotidyl Transferase(TdT)-mediated deoxyuridine triphosphate(d-UTP) nick and labeling method(TUNEL).Expression of CHOP/Gadd153,Bcl-2 were tested by immunohistochemistry and Western blot methods.Results: There were higher expressions of TUNEL-positve cells in the organ of Corti,stria vascularis,and spiral ganglion cells of experiment groups and significant differences were observed as compared with the control group(P<0.01).CHOP/Gadd153 expression was higher in 1-day,4-day and 14-day groups than that of the control(P<0.01),however,there was no statistically difference(P>0.05) among 1-day,4-day and 14-day groups.In the experimental groups,Bcl-2 was negatively expressed while it had significantly high expression(P<0.01) in the control group.Conclusion: Intense noise can lead to the apoptosis of guinea pig cochlea cells through increasing CHOP/Gadd153 protein expression and decreasing expression of Bcl-2 protein.