罗格列酮抗氧化应激效应影响3T3-L1细胞内脏脂肪素表达

目的:研究罗格列酮对3T3-L1细胞内脏脂肪素表达的影响及其机制。方法:体外培养并诱导分化3T3-L1细胞,加入葡萄糖激酶制作氧化应激模型,并用不同浓度和不同作用时间罗格列酮干预,观察脂肪因子表达的变化。结果:内脏脂肪素的表达随着葡萄糖激酶氧化应激的浓度增高而递减,具有剂量依赖效应(P<0.05);内脏脂肪素的表达随着罗格列酮干预浓度增加而增加(P<0.05),随着罗格列酮作用时间的延长,内脏脂肪素的表达经历了先下降后上升的过程,且罗格列酮对于内脏脂肪素表达的影响与氧化应激状态的改变平行。结论:罗格列酮可以通过抗氧化应激作用调节内脏脂肪素的表达,可能在罗格列酮改善肥胖相关的2型糖尿病胰岛素抵抗中起到重要作用。

Rosiglitazone Influences Expression of Visfatin in 3T3-L1 Adipocytes Through Inhibiting Oxidative Stress

Objective: To study the effect of rosiglitazone on 3T3-L1 adipocyte and the mechanisms. Methods: The 3T3-L1 adipocytes were cultured and induced to differentiation and maturity in vitro, and glucokinase was added to make an oxidative model. Then the adipocytes were treated by rosiglitazone at different doses and for different time. The expression of visfatin was determined by ELISA. Results: Visfatin expression was decreased along with the increase dose of the glucokinase in a concentration-dependent manner (P<0.05). With the pretreatment of oxidative stress, visfatin expression was increased according to the increasing doses of rosiglitazone (P<0.05). As the extension of rosiglitazone effect, expression of visfatin experienced decreasing-increasing process, and was parallel with the changes of oxidative stress. Conclusion: Rosiglitazone can increase visfatin expression in 3T3-L1 adipocyte by reducing oxidative stress, which could play a role in the treatment of insulin resistance in obesity related diabetes.