间接法ELISA检测流行性出血热抗体

用间接法ELISA检测流行性出血热IgG抗体。54份EHF病人血清全部阳性,32份正常人、19份发热病人和18份误诊为EHF病人的血清全部阴性,与IFA检测结果相同。检测EHF病人系列血清,发病3d就可检出抗EHF IgG,抗体滴度随病程升高,表明间接法ELISA可用于EHF临床诊断。将间接法ELISA与IFA、RPHI和HI平行检测30份EHF病人血清,抗体几何平均滴度(GMT)分别为1:43 211、1:413、1:253和1:143,有高度相关性,且ELISA比RPHI和HI更能检测出早期抗体。检测疫区人群血清4134份,间接法ELISA检出490份阳性,而IFA仅检出470份。结果表明间接法ELISA有较好的敏感度和特异度,尤其适用于血清流行病学研究。

DETECTION OF EPIDEMIC HEMORRHAGIC FEVER ANTIBODIES BY INDIRECT ELISA

An indirect ELISA test for IgG antibodies against EHF was established. Serum specimens collected from 54 EHF patients were all tested positive. 32 normal human serum specimens, 19 from patients with other diseases and 18 from patients whose illnesses had been misdiagnosed as EHF were all negative, These results were proved by IFA. IgG antibodies were detected in the EHF patients on the third day of illness, and the titers of the antibodies increased during the course of the disease. The GMTs of ELISA (1:43 211) was compared with IFA (1:413), EPHI (1:152) and HI (1:143). Significant correlations between indirect ELISA, IFA, EPHI and HI were observed. Examination of 4 134 serum specimens from the population in an endemic area revealed 490 true positives by indirect ELISA and 470 by IFA. These results demonstrated that indirect ELISA were more sensitive and of specific, and can be applied in clinical diagnosis, especially to seroepidemiologic studies.