大气飘浮颗粒物的有机提取物对雄小鼠生殖细胞的诱变作用

在火力发电厂下风侧居民区内收集大气颗粒物，经二氯甲烷提取，减压浓缩，橄榄油溶解作为受试物。给小鼠ｉｐ不同浓度受试物，观察精子畸形率，睾丸染色体畸变率和早期精细胞微核率的变化，以评价提取物对雄鼠生殖细胞的遗传毒效应。结果表明：５ｍｇ／ｋｇ抽取物对雄鼠生死细胞无明显诱变性；１０ｍｇ／ｋｇ以上可诱发精子畸形率显著高于阴性对照；２５ｍｇ／ｋｇ以上提取物可使精原细胞和初级精母细胞染色体畸变率明显升高，早期精

MUTAGENIC EFFECT OF EXTRACTS OF AMBIENT AIR FALL-OUT PARTICULATES ON GERM CELLS OF MALE MICE

Fallout particulates from ambient air were collected in an residential area downstream to the wind of a power plant.Then , extracted with dichloromethane,concentrated andredisolved in olive oil.The extract was administrated ip to mice at various dose. Sperm abnor-mality ,chromosome aberration of spermatogonia and primary speratocyte and meiotic mi-cronuclei of early spermatids were examined.The results showed that the extract in dose of5mg/kg exerted no significant mutagenicity on germ cells of male mice. The extract in doseon l0mg/kg and above induced sperm abnormality with frequency significantly higher thanthat of control;25mg/kg and above increased spermatogonia and primary spermatocyte chro-mosomal aberration and micronuclei frequency in early spermatids. These results indicatedthat the component of extract of fallout particulate can permeate blood-testis barrier intogerm cells of male mice and induced mutation on genetic material.