Studies on protective effect of DA-9601,Artemisia asiatica extract, on acetaminophen- and CCI4-induced liver damage in rats |
| |
Authors: | Byong Kweon Ryu Byoung Ok Ahn Tae Young Oh Soon Hoe Kim Won Bae Kim Eun Bang Lee |
| |
Institution: | (1) Research Laboratories, Dong-A Pharm. Co. Ltd., 47-5 Sanggalri, Kiheungup, Yonginshi, 449-900 Kyunggido, Korea;(2) Natural Products Institute, Seoul National University, Yongundong, Jongroku, 110-460 Seoul, Korea |
| |
Abstract: | The hepatoprotective effect of DA-9601, a quality-controlled extract ofArtemisia asiatica, on liver damage induced by acetaminophen (APAP) and carbon tetrachloride (CCI4) was investigated by means of serum-biochemical, hepatic-biochemical, and histopathological examinations. Doses of DA-9601
(10, 30, or 100mg/kg) were administered intragastrically to each rat on three consecutive days i.e. 48 h, 24 h and 2 h before
a single administration of APAP (640 mg/kg, i.p.) or CCl4 (2 ml/kg, p.o.). Four h and 24 h after hepatotoxin treatment, the animals were sacrificed for evaluation of liver damage.
Pretreatment of DA-9601 reduced the elevation of serum ALT, AST, LDH and histopathological changes such as centrilobular necrosis,
vacuolar degeneration and inflammatory cell infiltration dose-dependently. DA-9601 also prevented APAP- and CCl4-induced hepatic glutathione (GSH) depletion and CCl4-induced increase of hepatic malondialdehyde (MDA), a parameter of lipid peroxidation, in a dose-dependent manner. These findings
suggest that pretreatment with DA-9601 may reduce chemically induced liver injury by complex mechanisms which involve prevention
of lipid peroxidation and preservation of hepatic GSH. |
| |
Keywords: | DA-9601 Artemisia asiatica Hepatoprotection Acetaminophen Carbon tetrachloride Rat |
本文献已被 SpringerLink 等数据库收录! |
|