| Abstract: | Six kinds of antigens from the eggs and adult
worms of schistosoma japonicum were isolated. They
included adult microsomal antigen (JAMA), aqueous
soluble egg antigen (SEA), urea-soluble egg antigen
(JEU) and its further fractionated products (JECt-D.
They were analyzed by sodium polyacrylamide gel
electrophoresis (SDS-PAGE), enzyme-linked immuno-
electro-transfer blot (EITB) and kinetic-dependent
enzyme-linked immunosorbent assay (K-ELISA). They
were quantitatively assayed by K-ELISA for specific
antigenic activities against sera of 39 patients with
schistosomiasis japonica. The sera of patients with
diseases other than schistosomiasis and 10 healthy
adults were tested simultaneously as control. JEC:}
was antigenically more active (A A460/min X 10-j
than other antigens. Determinatin of the specific
antibody (IgG) levels of S. japonicum by using JEC:i,
etc as diagnostic antigens in 30 chronic patients re-
vealed that the level of anti-JEC:: antibody was the
highest (11.7_0.7A A460/min X 10-j.
EITB test showed tbat only JEC:; exhibited no
cross reactivity with sera from patients with parago
nimiasis and clonorchiasis sinensis, and that all posi-
tive bands of JEC; disappeared in patients cured of
chronic schistosomiasis. These findings suggest that
JEC:; might become a more satisfactory antigen in the
serodiagnosis of schistosomiasis japonica. |
