坤泰胶囊对初老雌性小鼠的戊巴比妥钠睡眠增强作用及其机制研究

目的评价坤泰胶囊对初老雌性小鼠戊巴比妥钠催眠作用的影响及其促睡眠机制。方法采用翻正反射试验考察坤泰胶囊0.8 g/kg对阈剂量戊巴比妥钠诱导的小鼠入睡潜伏期和睡眠时间的影响;采用RT-PCR技术检测坤泰胶囊0.8 g/kg对盐诱导激酶3(Sik3)和G蛋白偶联雌激素受体(Gper1)基因m RNA在不同睡眠状态下表达情况的影响。结果坤泰胶囊0.8 g/kg可以显著延长阈剂量戊巴比妥钠诱导小鼠的睡眠时间(P<0.05),缩短睡眠潜伏期(P<0.05);坤泰胶囊0.8 g/kg可升高视前区内Sik3 mRNA在6 h急性睡眠剥夺后的表达(P<0.05)。坤泰胶囊0.8 g/kg可一致性地降低大脑视前区和腹侧纹状体以及卵巢内Gper1 mRNA的表达,坤泰胶囊夺眠组显著降低腹侧纹状体和卵巢的雌激素受体基因Gper1 mRNA的表达(P<0.05)。结论坤泰胶囊可增强初老雌性小鼠的戊巴比妥钠催眠作用,其睡眠改善功效可能是通过提高Sik3和降低Gper1的表达而发挥作用。

Potentiating effect of Kuntai Capsules on sleep induced by pentobarbital sodium in primary and old female mice and its mechanism

Objective To study the effect of Kuntai Capsules on sleep induced by pentobarbital sodium in primary and old female mice and it''s mechanism. Methods The effect of Kuntai Capsules 0.8 g/kg on latency to persistent sleep and sleep time induced by pentobarbital sodium at threshold dose in mice and it''s possible mechanism by righting reflection test. The effect of Kuntai Capsules 0.8 g/kg on expression of Sik3 and Gper1 mRNA in different sleep states were detected by RT-PCR method. Results Kuntai Capsules 0.8 g/kg could significantly prolong sleep time induced by pentobarbital sodium at threshold dose in mice (P<0.05), and decrease sleep latency (P<0.05). Kuntai Capsules 0.8 g/kg could increase the expression of Sik3 mRNA in preoptic area after 6 h of acute sleep deprivation (P<0.05). Kuntai Capsules 0.8 g/kg could coincidently decrease the expression of Gper1 mRNA in preoptic area, ventral striatum, and ovary. Kuntai Capsules sleep-deprived group could significantly decrease the expression of Gper1 mRNA in ventral striatum and ovary (P<0.05). Conclusion Kuntai Capsules can increase sleep induced by pentobarbital sodium in primary and old female mice, and the sleep improvement effect is likely mediated by upregulating Sik3 and downregulating Gper1 mRNA expression.