芦丁通过抗氧化抗凋亡对蛛网膜下腔出血大鼠早期脑损伤的保护作用

目的探讨芦丁通过抗氧化抗凋亡对蛛网膜下腔出血(SAH)大鼠早期脑损伤的保护作用。方法颈内动脉穿刺法构建SAH模型。SD雄性大鼠分为假手术组、模型组和芦丁50 mg/kg组,24 h后对SAH评分、神经功能评分、脑含水量和伊文思蓝渗出率进行考察。并利用ELISA法检测氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活性;并采用免疫荧光检测调亡相关蛋白p53、Bax、caspase-3和Bcl-2的表达情况。结果与模型组比较,芦丁50mg/kg组SAH评分明显降低(P<0.05),神经功能评分明显提高(P<0.01)。与模型组比较,芦丁50mg/kg组大鼠左脑、右脑和小脑的脑水含量和伊文思蓝渗出率明显降低(P<0.05),提示芦丁能够减轻SAH大鼠血脑屏障的损伤。与模型组比较,芦丁50mg/kg组大鼠MDA水平显著降低(P<0.05),SOD、CAT和GSH-Px水平显著升高(P<0.05、0.01),提示芦丁具有改善SAH大鼠氧化应激的作用。芦丁50 mg/kg给药后p53、Bax和caspase-3表达量减少,Bcl-2表达增加。结论芦丁能够通过抗氧化抗凋亡减轻SAH大鼠的早期脑损伤。

Protective effect of rutin on early brain injury in mice with subarachnoid hemorrhage by anti-oxidation and anti-apoptosis

Objective To investigate the protective effect of rutin on early brain injury in mice with subarachnoid hemorrhage (SAH) by anti-oxidation and anti-apoptosis. Methods SAH model was established by internal carotid artery puncture. SD male mice were divided into Sham group, model group, and rutin 50 mg/kg group. After 24 h, SAH score, neurological function score, cerebral edema, and Evans blue exudation were investigated. The activities of oxidative stress indexes MDA, SOD, CAT, and GSH-Px were detected by ELISA method. The expression of apoptosis related proteins p53, Bax, caspase-3, and Bcl-2 were detected by immunofluorescence. Results Compared with the model group, SAH score in rutin 50 mg/kg group was significantly decreased (P<0.05), but neurological function score was significantly increased (P<0.01). Compared with the model group, cerebral edema and Evans blue exudation of left brain, right brain, and cerebellum in rutin 50 mg/kg group were significantly decreased (P<0.05), indicating that rutin could decrease the damage to the blood-brain barrier of mice with SAH. Compared with the model group, MDA level in rutin 50 mg/kg group was significantly decreased (P<0.05), but SOD, CAT, and GSH-Px levels were significantly increased (P<0.05, 0.01), indicating that rutin could improve the effect of oxidative stress in mice with SAH. Rutin 50 mg/kg could decrease the expression of p53, Bax, and caspase-3, and increase the expression of Bcl-2. Conclusion Rutin can alleviate early brain injury in mice with SAH by anti-oxidation and anti-apoptosis.