HPLC法同时测定栀子金花丸中栀子苷和黄岑苷的含量

目的建立HPLC法同时测定栀子金花丸中栀子苷和黄芩苷的含量测定方法。方法采用AgilentTC-C18色谱柱;甲醇-0.5%冰醋酸溶液为流动相,梯度洗脱;流速为0.8mL.min-1;检测波长为239nm。结果栀子苷和黄芩苷与其相邻杂质峰能完全分离,栀子苷在9.760～97.60μg.mL-1浓度范围内线性关系良好,r=0.9999;黄芩苷在10.42～104.2μg.mL-1浓度范围内线性关系良好,r=0.9999。栀子苷和黄芩苷平均回收率分别为100.56%、101.15%,RSD为2.57%和2.82%。结论本方法简便、准确、重复性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。

Simultaneous Determination of Geniposide and Baicalin in Zhizi Jinhua Pills by HPLC

Objective A HPLC method was established for determination of geniposide and baicalin in Zhizi Jinhua Pills.Methods Agilent TC-C18 column was used with the mobile phase of methanol-0.5% glacial acetic acid solution,gradient elution and the flow rate was 0.8mL·min-1.The detection wavelength was 239 nm.Results Geniposide and baicalin could be completely separated from interfacing impurity peaks.The linear range of geniposide was between 9.760～97.60 μg·mL-1,r=0.9999 and the linear range of baicalin was between 10.42～104.2μg·mL-1,r=0.9999.The average recoveries of geniposide and baicalin were 100.56%,101.15% respectively.RSD was 2.57%,2.82% respectively.Conclusion The methods is simple,accurate and reproducible.It can be used for the quality control of Zhizi Jinhua Pills.