胚胎干细胞视网膜下移植实验研究初步报告

目的 探讨胚胎干细胞(embronic stem　cell,ESC)在视网膜下间隙分化发育的情况。 方法 ESC经体外悬浮培养4 d形成胚胎体(embryonic body,EB)备作移植。将ESC以及EB联合或不联合视黄酸(retinoic acid,RA)分别移植到SD大鼠玻璃体腔或视网膜下，在视网膜下间隙移植眼，于移植前结扎眼动脉40 s造成缺血再灌注损伤的实验模型。于移植后14～28 d摘除眼球，观察移植物分化发育的情况。 结果 ESC在SD大鼠玻璃体腔内迅速增生，形成非典型性增生团块；EB在缺血再灌注损伤眼视网膜下间隙经RA诱导能定向分化为视网膜感光细胞；而在无RA作用下向多极化分化发育。单独RA视网膜下间隙注射后导致神经视网膜上皮增生变厚。 结论 ESC源性的EB在缺血再灌注眼视网膜下间隙经RA诱导能向视网膜感光细胞分化。（中华眼底病杂志，2000，16：213-284）

A preliminary report on experimental studies of embryonic stem cells transplanted into subretinal space

Purpose To investigate the development of embryonic stem cells (ESC)in the subretinal space． Methods ESC were cultivated in suspension for 4 days till they developed into cell aggregates,i.e.embryonic body(EB).ESC as well as　EB combined with or without RA were respectively transplanted into vitreous cavity and subretina1 space in SD rats,and the subretinal transplanted eyes,transient ischemia-reperfusion injuries were made by ligating the ophthalmic artery for 40 seconds before the transplantation .The experimental eyes were enucleated for histological and immunohistochemical assays after 14～28 d. Results The EB was found to develope into photoreceptors induced by RA in the subretinal space under an ischemia-reperfusion condition，and EB transplantation without RA induction induced multiple differentiations in the subretinal space.The single injection of RA without EB induced hyperplasia of the neural retinal cells.ESC transplanted into vitreous cavity rapidly proliferated and developed into atypical hyperplastic mass. Conclusion EB derived from ESC can differentiate into photoreceptors induced by RA in the host subretinal space under an ischemia-reperfusion condition.(Chin J Ocul Fundus Dis，2000，16：213-284)