中国南海聚裂丛柳珊瑚化学成分研究

目的研究聚裂丛柳珊瑚Rumphella aggregata的化学成分。方法利用硅胶色谱、Sephadex LH-20凝胶色谱、HPLC等手段对化学成分进行分离纯化;通过理化性质、波谱分析方法结合文献对照,鉴定化合物的结构。结果从聚裂丛柳珊瑚甲醇提取物中,共分离鉴定了15个单体化合物:胆甾醇(1)、(22E)-胆甾-5,22-二烯-3β-醇(2)、(22E)-麦角甾-5,22-二烯-3β-醇(3)、麦角甾-5,24(28)-二烯-3β-醇(4)、豆甾-5,24(28)-二烯-3β-醇(5)、柳珊瑚甾醇(6)、胆甾-7-烯-3β,5α,6β-三醇(7)、(22E)-胆甾-7,22-二烯-3β,5α,6β-三醇(8)、麦角甾-7-烯-3β,5α,6β-三醇(9)、(22E)-麦角甾-7,22-二烯-3β,5α,6β-三醇(10)、豆甾-7-烯-3β,5α,6β-三醇(11)、(22E)-豆甾-7,22-二烯-3β,5α,6β-三醇(12)、尿嘧啶(13)、咖啡因(14)、hydratoperidinin(15)。结论化合物1～15均为首次从该属柳珊瑚中分离得到,并首次对化合物hydratoperidinin(15)的1HNMR及13 CNMR信号进行了全归属。化合物12在10μg.mL-1浓度水平,对K562肿瘤细胞株的抑制率为39.17%。

Studies on chemical constituents of the South China Sea Gorgonian Rumphella aggregata

Objective To study the constituents of Rumphella aggregata.Methods The isolation and purification of the compounds were performed by silica gel,Sephadex LH-20 and HPLC methods,and their structures were determined by comparing their physicochemical characters and spectral data with literatures.Results From the methanol extracts of Rumphella aggregata.,fifteen compounds were isolated and indentified as cholesterol(1),(22E)-cholest-5,22-dien-3β-ol(2),(22E)-ergosta-5,22-diene-3β-ol(3),ergosta-5,24(28)-diene-3β-ol(4),stigmaster-5,24(28)-diene-3β-ol(5),gorgosterol(6),cholest-7-ene-3β,5α,6β-triol(7),cholest-7,22-diene-3β,5α,6β-triol(8),ergosta-7-ene-3β,5α,6β-triol(9),(22E)-ergosta-7,22-diene-3β,5α,6β-triol(10),stigmaster-7-ene-3β,5α,6β-triol(11),(22E)-stigmaster-7,22-diene-3β,5α,6β-triol(12),uracil(13),caffeine(14) and hydratoperidinin(15),respectively.Conclusion Compounds 1~15 were isolated from this coral for the first time,and compound 12 exhibited weak cytotoxicity against the K562 cell line with the inhibition rate 39.17% at the concentration level of 10 μg·mL-1.