| 半夏与其伪品掌叶半夏的RAPD鉴别 |
| |
| 引用本文： | 杨俊宝,彭正松.半夏与其伪品掌叶半夏的RAPD鉴别[J].现代中药研究与实践,2012(1):18-20. |
| |
| 作者姓名： | 杨俊宝 彭正松 |
| |
| 作者单位： | [1]川北医学院生物学教研室,四川南充637007 [2]西华师范大学生命科学学院,四川南充637002 |
| |
| 基金项目： | 四川省杰出青年基金后续项目（编号：05ZQ026-041） |
| |
| 摘 要： | 目的建立半夏及其伪品掌叶半夏的快速简单的分子鉴定方法。方法 SDS法提取半夏和掌叶半夏的基因组DNA,20个10碱基随机引物(S1-S20,上海生工)用于RAPD-PCR分析。结果 20个引物中有7个有效稳定的引物在半夏和掌叶半夏之间能显示出多态性指纹图谱,其中S10和S17在半夏和掌叶半夏之间显示较大差异。结论利用引物S10和S17进行RAPD-PCR能有效鉴别半夏和掌叶半夏。
|
| 关 键 词： | 半夏 掌叶半夏 指纹分析 RAPD |
Identification Pinellia ternata and Pinelliapedatisecta by RAPD |
| |
| YANG Jun-bao,PENG Zheng-song.Identification Pinellia ternata and Pinelliapedatisecta by RAPD[J].Research and Practice on Chinese Medicines,2012(1):18-20. |
| |
| Authors: | YANG Jun-bao PENG Zheng-song |
| |
| Institution: | 1. Department of Medical Biology, North Sichuan Medical College, Nanchong 637007, China; 2. College of Life Science, China West Normal University, Nanchong 637002, China ) |
| |
| Abstract: | Obiecfive To establish a rapid and simple molecular identification method for Pinellia ternata and Pinellia pedatisecta. Methods Genomic DNA was isolated from the dried tubers of P ternata and P pedatisecta by modified SDS method. Twenty 10-mer random primers （S1-S20） were used for RAPD analysis. Results Seven efficient and stable primers, showing distinct and polymorphic fingerprints, were selected from the 20 primers. The primers S 10 and S 17 were selected to differentiate Pinellia ternata from Pinellia pedatisecta. Conclusion RAPD analysis using the primer S10 and S17 would be accurate and reliable to identify P. ternata from P pedatisecta. |
| |
| Keywords: | Pinellia ternata P pedat＆ecta fingerprinting RAPD |
| 本文献已被 CNKI 维普 等数据库收录！ |
