气相色谱测定世界主产国西洋参人参中农药残留

目的检测世界主产国西洋参人参中六六六和滴滴涕7种异构体残留量。方法应用样品底物固相分散法,采用程序升温,进样温度250℃,检测器温度280℃,电子捕获检测器测定。结果西洋参人参中7种有机氯农药能有效分离,相关系数r在0.9 730~0.9 991,在3个添加水平的回收率均大于80%,相对标准偏差小于5%(n=6),检测限为0.5ng(S/N=3)。美国、加拿大、南北韩及我国各产地的西洋参人参中有机氯农药残留量很低,西洋参中的BHC为0.046~0.050μg.g-1、DDT为0.032~0.042μg.g-1;人参中的BHC为0.045~0.048μg.g-1、DDT为0.029~0.032μg.g-1。均低于标准BHC≤0.1mg.kg-1和DDT≤0.1mg.kg-1。结论世界主产国西洋参人参药材中有机氯农药残留量均符合2005年《中国药典》和中国药用植物及制剂进出口绿色行业标准规定。

Determination on Organochlorine Pesticide Residues in Radix Ginseng and Radix Panacis Quinquefolii from Main Production Countries in the world by Gas Chromatography

Objective To detect the residues of seven isomers of benzene hexachloride and chlorophenothane in Radix Ginseng and Radix Panacis Quinquefolii from main production countries in the world.Methods With the matrix solid-phase dispersion method（MSPD）,further purification was not necessary for subsequent gas chromatographic determination.The programmed temperature gas chromatography was adopted.The sampling temperature was 250℃ and the detector temperature was 280℃.The results were measured with the electron capture detector（ECD）.Results Seven isomers of the organochlorine pesticide could be efficiently separated in Radix Ginseng and Radix Panacis Quinquefolii.The correlation coefficient ＂r＂ was between 0.9730 and 0.9991.Each recovery rate under 3 adding levels was over 80%.The relative standard deviation was less than 5%（n=6）,and the detective limit was 0.5ng（S/N=3）.The organochlorine residues of the 4 samples of Radix Ginseng and Radix Panacis Quinquefolii from USA,Canada,south and north Korea were very low.BHC 0.046~0.050 μg？g-1 and DDT 0.032~0.042 μg？g-1 were detected in Radix Panacis Quinquefolii and BHC 0.045~0.048 μg？g-1 and DDT 0.029~0.032 μg？g-1 were detected in Radix Ginseng that were lower than the industrial criterion BHC≤0.1mg？kg-1 and DDT ≤0.1mg？kg-1.Conclusion The contents of organochlorine pesticide residues in Radix Ginseng and Radix Panacis Quinquefolii from main production countries in the world were in accordance with the content standard in Chinese Pharmacopoeia（2005 edition） and the criterion regulated by the import ＆ export green industry for Chinese medicinal herbs and preparation.