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缺氧缺血对O-2A祖细胞膜铁转运蛋白1表达的影响
引用本文:林清,张更,邱荣晖,王玮.缺氧缺血对O-2A祖细胞膜铁转运蛋白1表达的影响[J].解剖学报,2013,44(3):324-329.
作者姓名:林清  张更  邱荣晖  王玮
作者单位:福建医科大学基础医学院人体解剖学与组织学胚胎学系,神经生物学研究中心,福州 350108
基金项目:福建医科大学苗圃科研基金资助项目,省属高校专项科研基金资助项目
摘    要:目的 观察膜铁转运蛋白1(FPN1)在O-2A祖细胞缺氧缺血损伤后的表达变化,探讨其在O-2A祖细胞缺氧缺血损伤中的作用。方法 体
外培养O-2A祖细胞,以特异性抗体A2B5鉴定,观察FPN1在O-2A祖细胞上的定位表达;以糖氧剥夺(OGD)法建立缺氧缺血细胞模型,CCK8法检测
细胞存活率;应用免疫荧光染色法、实时荧光定量PCR法和Western blotting法观察FPN1在细胞缺氧缺血后的表达变化。结果 FPN1定位表达于
O-2A祖细胞的细胞膜、细胞质和突起;OGD3h、6h、12h及24h细胞存活率呈时间依赖性降低(P<0.05);OGD12h内细胞FPN1免疫荧光强度进行
性减弱;与OGD0h相比,FPN1 mRNA和蛋白水平在OGD3h表达下调,OGD6h进一步降低,OGD12h最低,差异具有统计学意义(P<0.05)。结论 O-2A
祖细胞缺氧缺血损伤后FPN1表达明显下调,细胞存活率显著降低,提示FPN1可能参与O-2A祖细胞的缺氧缺血损伤过程。

关 键 词:膜铁转运蛋白1    缺氧缺血    实时定量-聚合酶链反应    免疫印迹法    O-2A祖细胞
收稿时间:2012-08-13

Effect of hypoxia-ischemia on the expression of ferroportin 1 in O-2A progenitor cells
LIN Qing , ZHANG Geng , QIU Rong-hui , WANG Wei.Effect of hypoxia-ischemia on the expression of ferroportin 1 in O-2A progenitor cells[J].Acta Anatomica Sinica,2013,44(3):324-329.
Authors:LIN Qing  ZHANG Geng  QIU Rong-hui  WANG Wei
Institution:Department of Human Anatomy, Histology and Embryology, School of Preclinical Medicine,Research Center for Neurobiology, Fujian Medical University, Fuzhou 350108, China
Abstract:Objective To investigate the FPN1 expression and its role in O-2A progenitor cells after hypoxic-ischemic injury.
Methods O-2A progenitor cells were cultured in vitro, indentified with A2B5 antibody and investigated by the localization of
FPN1. Hypoxic-ischemic cell models were established by using the oxygen-glucose deprivation (OGD) method and the cell viability
was assessed by the CCK-8 method. The expression of FPN1 in O-2A progenitor cells after hypoxia-ischemia was detected by
immunofluorescent staining, quantitative real-time polymerase chain reaction analysis and Western blotting analysis. Results
FPN1 was localized at the cell membrane, and in the cytoplasm and processes of O-2A progenitor cells. The cell viability decreased
with time-dependence after 3hours, 6hours, 12hours and 24hours of OGD (P<0.05). The FPN1 immunofluorescence intensity of O-2A
progenitor cells decreased progressively within 12hours of OGD. The FPN1 mRNA and protein levels downregulated with time-dependence
after 3hours, 6hours and 12hours of OGD (P<0.05). Conclusion The level of FPN1 expression is down-regulated and cell viability
decreased significantly with time-dependence after OGD, which suggests that FPN1 may play a role in the hypoxic-ischemic injury of
O-2A progenitor cells.
Keywords:Ferroportin 1  Hypoxia-ischemia  Real-time PCR  Western blotting  Oligodendrocyte-type-2 astrocyte progenitor cell
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